GE17-5143-01
HiTrap® Benzamidine Fast Flow
Cytiva 17-5143-01, pack of 5 × 1 mL
About This Item
Produits recommandés
ligand
p-aminobenzamidine
Conditionnement
pack of 5 × 1 mL
Fabricant/nom de marque
Cytiva 17-5143-01
Conditions de stockage
(0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol)
Paramètres
42 psi
Taille du lit
7 mm × 25 mm
Volume du lit
1 mL
D.I. de la colonne
7 mm
Matrice
highly cross-linked 4% agarose
Diamètre moyen
90 μm
cleaning
1-9
working range
2-8
Température de stockage
2-8°C
Description générale
Application
The medium, Benzamidine Sepharose™ 4 Fast FLow (high sub), is also available as lab packages and is an excellent choice for scale-up. The columns can be operated using syringe, peristaltic pump or liquid chromatography system such as ÄKTAdesign or FPLC™ System.
Caractéristiques et avantages
- Effective removal of thrombin and factor Xa after tag cleavage of recombinant Proteins.
- Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates.
- HiTrap™ Benzamidine FF for convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein.
- High binding capacity.
- The columns can be operated using syringe, peristaltic pump or liquid chromatography system such as ÄKTA™ design or FPLC™ System.
Stockage et stabilité
Remarque sur l'analyse
Informations légales
Produit(s) apparenté(s)
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Code de la classe de stockage
3 - Flammable liquids
Certificats d'analyse (COA)
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Articles
This page shows troubleshooting strategies for cleavage methods using Cytiva products.
This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification of GST-tagged proteins in batch.
Protocoles
Protect fusion proteins from proteolytic degradation with enzyme removal pre- and post-cleavage using PreScission Protease, thrombin, or Factor Xa.
Protect fusion proteins from proteolytic degradation with enzyme removal pre- and post-cleavage using PreScission Protease, thrombin, or Factor Xa.
Protect fusion proteins from proteolytic degradation with enzyme removal pre- and post-cleavage using PreScission Protease, thrombin, or Factor Xa.
Protect fusion proteins from proteolytic degradation with enzyme removal pre- and post-cleavage using PreScission Protease, thrombin, or Factor Xa.
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