GE17-0599-01
Sephacryl® S-300 HR
Cytiva 17-0599-01, pack of 750 mL
Synonyme(s) :
Sephacryl S-300 Column
About This Item
Produits recommandés
Conditionnement
pack of 750 mL
Fabricant/nom de marque
Cytiva 17-0599-01
Matrice
allyl dextran and N, N’-methylenebisacrylamide
Taille des particules
25-75 μm
Taille moy. des particules
50 μm
cleaning in place
2-13
working range
3-11
Adéquation
suitable for bioprocess medium
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Catégories apparentées
Description générale
Sephacryl® High Resolution chromatography media is a highly versatile gel filtration media that offers a wide range of fractionation capabilities. The matrix of Sephacryl® High Resolution media is a cross-linked copolymer of allyl dextran and N,N′-methylene bisacrylamide. This crosslinking gives good rigidity and chemical stability. The narrow particle size distribution, together with steep selectivity curves, results in good preparative characteristics with maintained high resolution. The hydrophilic nature of the media minimizes nonspecific adsorption and maximizes recovery. The excellent resolution and flow characteristics, long-term physical and chemical stability, and ease of handling make Sephacryl® HR the medium of choice for routine purification.
Sephacryl® High Resolution is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As a BioProcess medium, Sephacryl® High Resolution, meets industrial demands with security of supply and comprehensive technical and regulatory support.
Five different Sephacryl® High Resolution chromatography media, with different fractionation ranges, are available:
Sephacryl® S-100 High Resolution is designed for separating peptides and small proteins (fractionation range globular proteins 1 × 103–1 × 105).
Sephacryl® S-200 High Resolution are for purifying antibodies, serum proteins and mid-size proteins (fractionation range globular proteins 5 × 103–2.5 × 105).
Sephacryl® S-300 High Resolution are for purifying antibodies, serum proteins and mid-size proteins (fractionation range globular proteins 1 × 104–1.5 × 106).
Sephacryl® S-400 High Resolution to separate polysaccharides, macromolecules with extended structures and even small particles such as plasmids. (fractionation range globular proteins 2 × 104–8 × 106).
Sephacryl® S-500 High Resolution to separate polysaccharides, macromolecules with extended structures and even small particles such as plasmids (fractionation range Dextrans 4 × 104–2 × 107)
Caractéristiques et avantages
- Cost-efficient gel filtration media providing high resolution with fast and easy operation.
- Five different fractionation ranges available in Sephacryl® HR family allows for reproducible separations over a wide molecular weight range.
- Sephacryl® S-300 High Resolution medium is designed for purifying antibodies, serum proteins and mid-size proteins (fractionation range globular proteins 1 × 104–1.5 × 106)
- BioProcess medium supported for industrial applications and well-established in approved processes.
Stockage et stabilité
Remarque sur l'analyse
Informations légales
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Code de la classe de stockage
3 - Flammable liquids
Certificats d'analyse (COA)
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Protocoles
This page provides information about performing an isolation of natural multi-protein complexes for structural and functional studies using classical biochemical methods and products from Cytiva.
Sephacryl column separates using Size Exclusion Chromatography media, prepared by covalently cross-linking allyl dextran.
Sephacryl column separates using Size Exclusion Chromatography media, prepared by covalently cross-linking allyl dextran.
Sephacryl column separates using Size Exclusion Chromatography media, prepared by covalently cross-linking allyl dextran.
Contenu apparenté
Separation options for Size Exclusion Chromatography with Sephacryl from Cytiva.
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