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F0162

Sigma-Aldrich

Fibronectine

lyophilized powder, 45 kDa

Synonyme(s) :

Fibronectin

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About This Item

Numéro MDL:
MFCD00131062
Code UNSPSC :
12352202
eCl@ss :
42020141
Nomenclature NACRES :
NA.75

Source biologique

human plasma

Niveau de qualité

200

Pureté

≥90% (SDS-PAGE)

Forme

lyophilized powder

Poids mol.

45 kDa

Conditionnement

pkg of 0.5 mg

Technique(s)

cell culture | mammalian: suitable

Impuretés

HIV and HBsAg, source material tested negative
 Small proteolytic fragments, may contain traces

Solubilité

water: soluble ≥0.500 mg/mL, clear to slightly hazy, colorless

Numéro d'accès UniProt

P02751

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

human ... FN1(2335)

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Description générale

Fibronectin 1 is a glycoprotein of the extracellular matrix that is coded by FN1 gene. It is expressed in the plasma and at the cell surface.FN1 is mapped to human chromosome 2q35. Proteolytic fragments of fibronectin has a cell binding domain. Plasma fibronectin has two polypeptide chains connected by two disulphide bonds present near carboxy terminal. Each polypeptide chain is of 220-250 kDa.

Application

Fibronectin proteolytic fragment from human plasma has been used as fluorophore-conjugated proteins. It has also been used in solid phase binding assay.

Actions biochimiques/physiologiques

Fibronectin participates in cell adhesion, growth, migration, wound healing, blood coagulation and metastasis. Mutations in FN1 results in glomerulopathy. It plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in extracellular matrix formation, hemostasis and thrombosis. Proteolytic fragments of fibronectin plays a vital role in mononuclear phagocyte function.
Fibronectins are high molecular weight glycoproteins with two subunits joined by a disulfide bond to form the dimer. The fragments are obtained using protelytic enzymes. This 45 kDa gelatin binding fragment is obtained through trypitc digestion of the N-terminal 70 kDa fragment, which is produced by Cathespin D digestion.

This fragment has an acidic pI (4.9-5.3) and does not bind to heparin. This domain is resistant to proteolysis due to intrachain disulfide bonding and the attached carbohydrate. The intrachain disulfide bonds are essential for binding to gelatin, while the complex, branched, asparagine-linked carbohydrate is not. This fragment binds to C1q, but not to fibrin.

Attention

The product should be stored at -20°C.

Notes préparatoires

This product is lyophilized from phosphate buffered saline with sucrose as a cryoprotectant. The source material has tested negative for antibody to HIV, HCN, and HBsAg. It is soluble in water at 0.5 mg/mL and yields a clear to slightly hazy solution.

En option

Réf. du produit
Description
Tarif

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H315,H319,H335

Classification des risques

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Fibronectin in Health and Disease (2018)
Nanoparticle amplification via photothermal unveiling of cryptic collagen binding sites.
Lo J H, et al.
Journal of Material Chemistry B: Materials for Biology and Medicine, 1(39), 5235-5240 (2013)
Justin H Lo et al.
Journal of materials chemistry. B, 1(39), 5235-5240 (2013-11-02)
The success of nanoparticle-based cancer therapies ultimately depends on their ability to selectively and efficiently accumulate in regions of disease. Outfitting nanoparticles to actively target tumor-specific markers has improved specificity, yet it remains a challenge to amass adequate therapy in
Samadrita Bhattacharyya et al.
The Journal of clinical investigation (2022-12-02)
Comprehensive cis-regulatory landscapes are essential for accurate enhancer prediction and disease variant mapping. Although cis-regulatory element (CRE) resources exist for most tissues and organs, many rare - yet functionally important - cell types remain overlooked. Despite representing only a small
A R Pickford et al.
Structure (London, England : 1993), 5(3), 359-370 (1997-03-15)
Fibronectin is an extracellular matrix glycoprotein involved in cell adhesion and migration events in a range of important physiological processes. Aberrant adhesion of cells to the matrix may contribute to the breakdown of normal tissue function associated with various diseases.
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Protocoles

Protocole de coating de fibronectine

Diluer la fibronectine à la concentration souhaitée. Les conditions d'adhésion optimales dépendent du type de cellules et de l'application. La concentration de coating standard varie de 1 à 5 µg/cm2. Protocole de coating de fibronectine, produits et FAQ.

Protocole de coating de fibronectine

Diluer la fibronectine à la concentration souhaitée. Les conditions d'adhésion optimales dépendent du type de cellules et de l'application. La concentration de coating standard varie de 1 à 5 µg/cm2. Protocole de coating de fibronectine, produits et FAQ.

Protocole de coating de fibronectine

Diluer la fibronectine à la concentration souhaitée. Les conditions d'adhésion optimales dépendent du type de cellules et de l'application. La concentration de coating standard varie de 1 à 5 µg/cm2. Protocole de coating de fibronectine, produits et FAQ.

Fibronectin Coating Protocol

Dilute fibronectin for cell attachment, varying per cell type. Coating protocol, products, and FAQs provided.

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