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650498

Sigma-Aldrich

Chloroforme

HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%, contains amylenes as stabilizer

Synonyme(s) :

Trichlorométhane, Trichlorure de méthylidyne

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About This Item

Formule empirique (notation de Hill):
CHCl3
Numéro CAS:
Poids moléculaire :
119.38
Numéro Beilstein :
1731042
Numéro CE :
Numéro MDL:
Code UNSPSC :
12190000
ID de substance PubChem :
Nomenclature NACRES :
NA.04

Qualité

HPLC Plus
for residue analysis

Niveau de qualité

Densité de vapeur

4.1 (vs air)

Pression de vapeur

160 mmHg ( 20 °C)

Pureté

≥99.9%

Forme

liquid

Contient

amylenes as stabilizer

Technique(s)

HPLC: suitable
gas chromatography (GC): suitable

Impuretés

≤0.02% water
≤0.5 ppb Fluorescence (quinine) at 365 nm

Résidus d'évap.

≤0.0001%

Résidus halogénés

<10 ng/L (as heptachlor epoxide)

Indice de réfraction

n20/D 1.445 (lit.)

Point d'ébullition

60.5-61.5 °C (lit.)

Pf

−63 °C (lit.)

Densité

1.48 g/mL at 25 °C
1.492 g/mL at 25 °C (lit.)

λ

H2O reference

Absorption UV

λ: 245 nm Amax: 1.00
λ: 250 nm Amax: 0.30
λ: 275-400 nm Amax: 0.005

Application(s)

food and beverages

Chaîne SMILES 

ClC(Cl)Cl

InChI

1S/CHCl3/c2-1(3)4/h1H

Clé InChI

HEDRZPFGACZZDS-UHFFFAOYSA-N

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Description générale

Chloroform is an organic chlorinated hydrocarbon (CHC) commonly used in industries as a solvent.

Application

Chloroform may be used in the following:

  • Extraction of active constituents of anti-inflammatory drugs for their analysis by attenuated total reflectance-Fourier transform infrared spectroscopic (ATR-FTIR) methods
  • Estimation of ascorbic acid, free phenolic acids, and flavonoids in extracts of chloroform, methanol, water and, acetic acid obtained from two edible leaf samples using reversed-phase high-performance liquid chromatography (RP-HPLC) coupled to photodiode array detector (DAD)
  • Preparation of cannabis product extracts for the analysis of delta-9-tetrahydrocannabinol (THC) residues on hands of suspected consumers by ion-mobility spectrometry (IMS)
  • Investigation of different solvents to measure their recovery ratios for the extraction of acrylamide and 5-hydroxymethylfurfural (HMF) from seafood samples to develop an HPLC-MS/MS-based method for their quantification
  • Extraction of pesticides by direct-immersion single drop micro-extraction (SDME) method from mango samples for their subsequent analysis by GC-MS
  • Sample preparation in the profiling of lipids from complex biological samples using off-line mixed-mode liquid chromatography coupled with reversed phase high performance liquid chromatography-high resolution mass spectrometry

Chloroform was used in the making of silanized coverslips for DNA combing.
It may be used as:
  • Extractant in solvent extraction process.
  • A component of the binary mobile phases for liquid chromatography–atmospheric pressure photoionization-mass spectrometry (LC–APPIMS) of triacylglycerol model compounds.
  • Solvent for recrystallization.
Suitable for HPLC, spectrophotometry, environmental testing

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Pictogrammes

Skull and crossbonesHealth hazard

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 3

Organes cibles

Central nervous system, Liver,Kidney

Code de la classe de stockage

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

does not flash

Point d'éclair (°C)

does not flash


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In this work, we evaluate the performance of liquid chromatography-atmospheric pressure photoionization-mass spectrometry (LC-APPI-MS) for non-aqueous reversed phase analysis of six triacylglycerol model compounds using six binary mobile phases including MeOH/iPrOH, MeOH/CHCl(3), MeOH/CH(2)Cl(2), CH(3)CN/iPrOH, CH(3)CN/CHCl(3), and CH(3)CN/CH(2)Cl(2). All mobile phases
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