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B8522

Sigma-Aldrich

Brilliant Blue G solution

Concentrate

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About This Item

Numéro CAS:
6104-58-1
Numéro MDL:
MFCD00078482
Code UNSPSC :
12171500
ID de substance PubChem :
24892075
Nomenclature NACRES :
NA.47

Forme

liquid

Couleur

dark blue

Adéquation

suitable for (Suitable for for staining protein in polyacrylamide and agarose gels)

Application(s)

diagnostic assay manufacturing
hematology
histology

Température de stockage

2-8°C

Chaîne SMILES 

[Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3C)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=CC(\C=C5C)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1

InChI

1S/C47H49N3O7S2.Na/c1-6-49(31-35-11-9-13-43(29-35)58(51,52)53)40-21-25-45(33(4)27-40)47(37-15-17-38(18-16-37)48-39-19-23-42(24-20-39)57-8-3)46-26-22-41(28-34(46)5)50(7-2)32-36-12-10-14-44(30-36)59(54,55)56;/h9-30H,6-8,31-32H2,1-5H3,(H2,51,52,53,54,55,56);/q;+1/p-1

Clé InChI

RWVGQQGBQSJDQV-UHFFFAOYSA-M

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Catégories apparentées

Description générale

Brilliant Blue G is a methanol based stain which is especially designed for staining protein in polyacrylamide gels, but will also stain protein in agarose gels (IEF). The stain contains methanol and acetic acid so gels do not require fixing prior to staining. Water is added directly to the bottle to produce 1 liter of ready-to-use staining solution.

Application

Brilliant Blue G solution has been used for protein band detection after SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). It is also used for the protein staining of cells on microscopic slide.

Quantité

One bottle equals one liter working solution. 300 ml of brilliant blue G solution concentrate is supplied in the bottle.

Reconstitution

After dilution with deionized water to a final volume of one liter, solution will contain 0.1% (w/v) Brilliant Blue G, 25% (v/v) methanol and 5% (v/v) acetic acid.

Remarque sur l'analyse

This product has been tested for suitability on SDS-PAGE.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

G1041

EZBlue™ Gel Staining Reagent

Point d'éclair (°F)

49.5 °F - closed cup

Point d'éclair (°C)

9.7 °C - closed cup

Équipement de protection individuelle

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
SLCN1989
SLCK9977
SLCF2517
SLBX7219
SLBS7637

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Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

Rechercher un(e) COA

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Emerin suppresses Notch signaling by restricting the Notch intracellular domain to the nuclear membrane.
Biochimica et Biophysica Acta, 1864, 303-303 (2017)
Ahmed H
Principles and Reactions of Protein Extraction, Purification, and Characterization (2004)
Effect of an experimental proteasome inhibitor on the cytoskeleton, cytosolic protein turnover, and induction in the neuronal cells in vitro.
Csizmadia V, et al.
Neurotoxicology, 29, 232-232 (2008)
Suma Tiruvayipati et al.
PloS one, 15(3), e0228871-e0228871 (2020-03-03)
Exosomes are extracellular vesicles (EVs) of ~20-200 nm diameter that shuttle DNAs, RNAs, proteins and other biomolecules between cells. The large number of biomolecules present in exosomes demands the frequent use of high-throughput analysis. This, in turn, requires technical replicates
Starlee Lively et al.
Frontiers in cellular neuroscience, 12, 433-433 (2018-12-14)
Addressing potential sex differences in pre-clinical studies is crucial for developing therapeutic interventions. Although sex differences have been reported in epidemiological studies and from clinical experience, most pre-clinical studies of neuroinflammation use male rodents; however, sexual dimorphisms in microglia might
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