B6529

Brilliant Blue R Staining Solution

ethanol solution

• Synonyme(s) : Brilliant Blue R, Acid Blue 83, Brilliant indocyanin 6B, Coomassie Brilliant Blue R
• Formule empirique (notation de Hill): C₄₅H₄₄N₃NaO₇S₂
• Numéro CAS: 6104-59-2
• Poids moléculaire : 825.97
• Numéro C.I. (Colour Index): 42660
• Numéro Beilstein : 5718025
• Numéro MDL: MFCD00041762
• Code UNSPSC : 12171500
• ID de substance PubChem : 24891914
• Nomenclature NACRES : NA.47

Propriétés

• product name: Brilliant Blue R Staining Solution, suitable for (for immunoelectrophoresis protein staining)
• Forme: liquid
• Niveau de qualité: 200
• Couleur: dark blue
• Adéquation: suitable for (for immunoelectrophoresis protein staining)
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: room temp
• Chaîne SMILES: [Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=C/C(C=C5)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1
• InChI: 1S/C45H45N3O7S2.Na/c1-4-47(31-33-9-7-11-43(29-33)56(49,50)51)40-23-15-36(16-24-40)45(35-13-19-38(20-14-35)46-39-21-27-42(28-22-39)55-6-3)37-17-25-41(26-18-37)48(5-2)32-34-10-8-12-44(30-34)57(52,53)54;/h7-30H,4-6,31-32H2,1-3H3,(H2,49,50,51,52,53,54);/q;+1/p-1
• Clé InChI: NKLPQNGYXWVELD-UHFFFAOYSA-M

Description

Application

Brilliant Blue R staining solution is especially designed for use in staining protein in agarose gels following immunoelectrophoresis and Ouchterlony gels. The stain contains ethanol and acetic acid so gels do not require fixing prior to staining. The immunoelectrophoresis or Ouchterlony gel is first washed with water and saline to remove non-precipitated proteins and then dried. The gel is then immersed in staining solution for 30 min and destained with 10% acetic acid.

For detection of protein bands following electrophoresis.

Composants

0.5% (w/v) Brilliant Blue R, 45% (v/v) ethanol and 10% (v/v) acetic acid.

Remarque sur l'analyse

Tested for suitability on agarose gels following immunoelectrophoresis.

Informations légales

Informations sur la sécurité

• Point d'éclair (°F): 81.0 °F - closed cup
• Point d'éclair (°C): 27.2 °C - closed cup
• Équipement de protection individuelle: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter