B6916
Synonyme(s) :
Dosage des protéines par liaison du bleu de Coomassie, réactif de coloration des protéines
| À vous/Référence | Disponibilité | Prix |
|---|
1 of 1
Cet article | |||
|---|---|---|---|
| form solution | form - | form - | form - |
| Quality Level 200 | Quality Level 100 | Quality Level 200 | Quality Level 200 |
| storage temp. 2-8°C | storage temp. - | storage temp. - | storage temp. −20°C |
Warning
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 2
Eyes,Central nervous system
Not applicable
Not applicable
12 - Non Combustible Liquids
Faites votre choix parmi les versions les plus récentes :
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
For the Standard 3.1 mL Assay and 96 Well Plate Assay, the protein standards should be prepared in the range of 0.1 - 1.4 mg/mL. This is not the final concentration of the protein after being diluted in the assay.
For the full protocol, please refer to the document found here:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/165/479/b6916bul-ms.pdf
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Yes, phenol red at concentrations above 0.5 mg/mL will cause interference. Concentrations below 0.5 mg/mL are expected to be compatible.
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The color of this product can range from Faint Yellow-Brown to Light Brown. The exact result will be listed on the lot specific Certificate of Analysis. Please navigate to the ‘DOCUMENTATION’ section of the Product Detail Page to access a Certificate under ‘Certificate of Analysis’: https://www.sigmaaldrich.com/product/sigma/b6916#product-documentation
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A compatibility chart is listed in the bulletin. This chart indicates that this reagent is compatible with concentrations of SDS up to 0.125%.
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The Bradford method has a lower limit of detection of 20 ug/mL. The Lowry method has a lower limit of 10 ug/mL. Concentration of the sample may be necessary.
See the links below for additional helpful information:
Protein Quantitation Methods-
https://www.sigmaaldrich.com/applications/protein-biology/protein-quantitation
Amicon Centrifugal Filters-
https://www.sigmaaldrich.com/technical-documents/technical-article/protein-biology/protein-concentration-and-buffer-exchange/amicon-ultra-centrifugal-filters
Centricon Centrifugal Filters-
https://www.sigmaaldrich.com/substance/centriconplus70centrifugalfilter1234598765
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Albumins are readily soluble in water and can only be precipitated by high concentrations of neutral salts such as ammonium sulfate. The solution stability of BSA is very good (especially if the solutions are stored as frozen aliquots). In fact, albumins are frequently used as stabilizers for other solubilized proteins (e.g., labile enzymes). However, albumin is readily coagulated by heat. When heated to 50°C or above, albumin quite rapidly forms hydrophobic aggregates which do not revert to monomers upon cooling. At somewhat lower temperatures aggregation is also expected to occur, but at relatively slower rates.
Please see the product data sheet which describes the solution stability of BSA:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/351/531/a8412pis.pdf
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the useful concentration range that can be measured by Product B6916, Bradford Reagent?
The Bradford Reagent requires no dilution and is suitable for micro, multiwell plate, and standard (cuvet) assays. The linear concentration range is 0.1-1.4 mg/mL of protein, using BSA (bovine serum albumin) as the standard protein.
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When using Product B6916, Bradford Reagent, what can I do if I have a very dilute sample in a large volume?
The micro assay using this same reagent may be an option for you. The micro assay is used when a large volume (at least 1 mL) of a dilute sample is available for testing. The linear concentration range of this assay is lower than the standard or multiwell plate assays, (1-10 μg of total protein in 1 mL).
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Will the buffer or solution my protein is in interfere with Product B6916, Bradford Reagent?
A compatibility chart is listed in the bulletin. If your substance is not listed, then we recommend testing this by diluting the standard protein samples in the same buffer as the unknown samples.
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