11579681001
Roche
RNase, DNase-free, High Concentration
from bovine pancreas
Synonyme(s) :
Rnase
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About This Item
Code UNSPSC :
41105600
Produits recommandés
Source biologique
bovine pancreas
Niveau de qualité
Forme
solution
Activité spécifique
≥30 U/mg
Conditionnement
pkg of 1 mg (10 mg/ml)
Fabricant/nom de marque
Roche
Concentration
10 mg/mL
Description générale
A heterogeneous mixture of ribonucleases specially prepared to be free of deoxyribonuclease activity according to the current Quality Control procedures.
Application
High concentration, DNase-free RNase has been used for:
- Genomic DNA isolation
- Isolation of plasmid DNA
- Degradation of RNA for flow cytometry
Qualité
Absence of DNase activity tested according to the current Quality Control procedures.
Définition de l'unité
One unit is the enzyme activity that causes a decrease in absorbance of A0 to A1 within 1 minute under assay conditions: A0 to A1 corresponds to the total conversion, A1 being the final absorbance.
Notes préparatoires
Working concentration: 50 μg/ml is the recommended working concentration of RNase, DNase-free, for the isolation of genomic DNA.
Working solution: Recommended in dilution buffer: 10 mM Tris-HCl, 5 mM CaCl2, 50% glycerol (v/v), pH 7.0
Working solution: Recommended in dilution buffer: 10 mM Tris-HCl, 5 mM CaCl2, 50% glycerol (v/v), pH 7.0
Autres remarques
For life science research only. Not for use in diagnostic procedures.
Unlike most RNase preparations, no boiling before use is necessary to remove DNase activity.
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Resp. Sens. 1
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
does not flash
Point d'éclair (°C)
does not flash
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