MABD398

Anti-DAX-1, clone 2F4 Antibody

ascites fluid, clone 2F4, from mouse

• Synonyme(s) : Dax1, Nuclear receptor subfamily 0 group B member 1, DSS-AHC critical region on the X chromosome protein 1, Nuclear receptor DAX-1, AHC
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: ascites fluid
• Type de produit anticorps: primary antibodies
• Clone: 2F4, monoclonal
• Espèces réactives: human
• Technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_000466
• Numéro d'accès UniProt: P51843
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... NR0B1(190)

Description

Description générale

DAX-1 is an orphan nuclear receptor and is required for the normal development of the hypothalamic-pituitary-adrenal-gonadal axis formation. Defects in DAX-1 are the cause of X-linked adrenal hypoplasia congenital (XL-AHC); also known as X-linked Addison disease (AHX). DAX-1 also has a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency by acting as a negative regulator of Oct3/4 in ES cells.

Immunogène

Linear peptide corresponding to Human DAX-1 (amino acids 135-166).

Application

Anti-DAX-1, clone 2F4, has been shown to work in Immunocytochemical (Lehmann et al., 2009) and Immunohistochemical (Ehrlund et al., 2013) analysis.

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

This Anti-DAX-1, clone 2F4 Antibody is validated for use in Western Blotting and Immunocytochemistry and Immunohistochemistry for the detection of DAX-1.

Qualité

Evaluated by Western Blot in a Dax-1 overexpressing HEK293 transfected cell line. Western Blot Analysis: A 1:1000 dilution of this antibody detected DAX-1 in a DAX-1 overexpressing HEK293 transfected cell line.

Description de la cible

~ 52 kDa observed

Liaison

Replaces: 04-445

Forme physique

Mouse monoclonal IgG1κ Ascites with 0.05% sodium azide.

Unpurified

Stockage et stabilité

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable