P4458

Pénicilline-streptomycine

liquid, non-animal origin, suitable for cell culture, BioReagent

• Synonyme(s) : Pen-Strep
• Code UNSPSC : 12352207
• Nomenclature NACRES : NA.76

Propriétés

• Nom du produit: Pénicilline-streptomycine, Solution Stabilized, with 5,000 units penicillin and 5mg streptomycin/mL, 0.1 μm filtered, BioReagent, suitable for cell culture
• Niveau de qualité: 300
• Stérilité: 0.1 μm filtered
• Gamme de produits: BioReagent
• Forme: liquid
• Concentration: 50 ×
• Technique(s): cell culture | mammalian: suitable
• Impuretés: endotoxin, tested
• Spectre d'activité de l'antibiotique: Gram-negative bacteria; Gram-positive bacteria
• Mode d’action: cell wall synthesis | interferes; protein synthesis | interferes
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Chaîne SMILES: CC1(C)S[C@@H]2[C@H](NC(=O)Cc3ccccc3)C(=O)N2[C@H]1C(O)=O.CNC[C@H]4[C@H](O)[C@@H](O)[C@H](CCO)O[C@H]4O[C@H]5[C@@H](O[C@@H](C)C5(O)C=O)O[C@H]6[C@H](O)[C@@H](O)[C@H](NC(N)=N)[C@@H](O)[C@@H]6NC(N)=N
• InChI: 1S/C23H43N7O12.C16H18N2O4S/c1-7-23(38,6-32)18(42-19-8(5-28-2)12(33)13(34)9(40-19)3-4-31)20(39-7)41-17-11(30-22(26)27)14(35)10(29-21(24)25)15(36)16(17)37;1-16(2)12(15(21)22)18-13(20)11(14(18)23-16)17-10(19)8-9-6-4-3-5-7-9/h6-20,28,31,33-38H,3-5H2,1-2H3,(H4,24,25,29)(H4,26,27,30);3-7,11-12,14H,8H2,1-2H3,(H,17,19)(H,21,22)/t7-,8-,9-,10+,11-,12-,13-,14+,15-,16+,17+,18-,19-,20-,23+;11-,12+,14-/m01/s1
• Clé InChI: KMOOCZWLFBSQCW-XFOLRDBHSA-N

Description

Description générale

Penicillin, a β-lactam antibiotic is extremely active towards bacteria.[1] Penicillins are also termed as penams. It is naturally occurring and has a bicyclic skeleton.[2] Streptomycin is a pseudotrisaccharide. It comprises of a monosubstituted aminocyclitol.[3]

Application

Penicillin-Streptomycin has been used:

• as a supplement in Dulbecco′s modified Eagle′s medium (DMEM) to maintain the Huh7 liver hepatoma cell line[4]
• as a supplement in Roswell park memorial institute-1640 (RPMI) medium to maintain mouse lymphoma YAC-1 cells[5]
• as a component in minimum essential medium Eagle to treat individual mouse spleens for the preparation of splenocytes[6]
• as a supplement in FMX-8 complete medium to culture Chinese hamster ovary cells[7]

Recommended for use in cell culture media at 20 ml/L.

Actions biochimiques/physiologiques

Penicillins can prevent the synthesis of bacterial cell wall. This antibiotic can be used in the treatment of skin and urinary tract infections, upper and lower respiratory infections, and endocarditis.[8] Streptomycin can block Mycobacterium tuberculosis in vitro.[4] Streptomycin inhibits prokaryote protein synthesis[7] by preventing the transition from initiation complex to chain-elongating ribosome, causing miscoding.

Composants

This product is formulated to contain 5,000 units penicillin and 5 mg streptomycin/mL solubilized in a proprietary citrate buffer.

Autres remarques

20ml,100ml

Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable