This product contains albumin; the albumin value is lot-specific and listed on the Certificate of Analysis. Please see the link below to access a lot-specific Certificate:
https://www.sigmaaldrich.com/product/sigma/f7524#product-documentation
F7524
Sérum de veau fœtal
non-USA origin, sterile-filtered, suitable for cell culture
Fetal Bovine Serum
1 of 8 reviewers received a sample product or took part in a promotion
Synonyme(s) :
FBS, FCS, sérum, sérums
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A propos de cet article
Biological source:
bovine fetus
Origin:
non-USA origin
Sterility:
sterile-filtered
Shipped in:
dry ice
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Service technique
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Laissez-nous vous aiderbiological source
bovine fetus
Quality Segment
sterility
sterile-filtered
product line
FBS Classic
composition
hemoglobin, ≤25 mg/dL
origin
non-USA origin
availability
not available in Canada, USA
technique(s)
cell culture | mammalian: suitable
impurities
≤10 EU/mL endotoxin
shipped in
dry ice
storage temp.
−20°C
Application
Le sérum de veau fœtal a été utilisé comme supplément dans des milieux de culture cellulaire tels que le milieu RPMI-1640 (développé au Roswell Park Memorial Institute) et le milieu de Eagle modifié par Dulbecco (DMEM)[1] afin de faciliter la croissance de diverses lignées cellulaires.
Biochem/physiol Actions
Le sérum de veau fœtal (FBS) est un supplément pour milieu de culture cellulaire couramment utilisé pour faciliter la croissance in vitro et ex vivo des cultures de cellules, de tissus et d'organes. Le FBS reste un supplément pour milieux très prisé, car il assure de multiples fonctions en culture cellulaire. Il apporte des nutriments, des facteurs de croissance et d'adhésion et protège les cellules des dommages oxydatifs et de l'apoptose par des mécanismes qui sont difficiles à reproduire avec les milieux sans sérum. Il apporte aux cellules en culture de nombreux facteurs de croissance et de survie non définis. Le FBS dérivé de sang coagulé est le supplément non défini le plus utilisé dans la culture de cellules eucaryotes, et notamment de cellules de mammifères. Il est généralement utilisé avec les milieux classiques mis au point dans les années 1950 et 1960. Ces derniers comprennent de nombreux milieux très prisés, comme le milieu essentiel minimum (MEM), le milieu d'Eagle modifié par Dulbecco (DME ou DMEM), le milieu de Dulbecco modifié par Iscove (IMDM), le milieu M199 et le milieu RPMI 1640 (Roswell Park Memorial Institute).
Features and Benefits
F7524 est le même excellent produit que le produit S0115 de Biochrom GmbH.
Analysis Note
- A fait l'objet de tests pour rechercher la présence de bactéries, de virus et de mycoplasmes
- Filtré trois fois sur membrane de 0,1 micron en conditions aseptiques
- Taux d′endotoxines et d′hémoglobine testés
Legal Information
Non disponible aux États-Unis et au Canada. Veuillez vérifier auprès de votre fournisseur local la disponibilité et les restrictions d'importation dans votre pays.
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Cet article | |||
|---|---|---|---|
| biological source bovine fetus | biological source bovine fetus | biological source bovine fetus | biological source bovine fetus |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| origin non-USA origin | origin Australia origin | origin USA origin | origin USA origin |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
| impurities ≤10 EU/mL endotoxin | impurities ≤10 EU/mL endotoxin | impurities ≤10 EU/mL endotoxin (typically ≤1 EU/ml) | impurities ≤10 EU/mL endotoxin |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
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Classe de stockage
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Guten Morgen Enthält das gekaufte Kälberserum Albumin ?
1 answer-
Helpful?
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Do we still need to inactivate it via heat protection?
1 answer-
This product has not been heat inactivated. Should heat inactivation be required for specific culture purposes, the bottles may be incubated at 56°C for 30 minutes. Allow serum to reach room temperature and swirl bottles to mix well, prior to placing in the water bath. Swirl to mix every 5 - 10 minutes during the 30 minute incubation period.
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Does the F7524 Fetal Bovine Serum product contain Tetracycline?
1 answer-
Catalog number F7524 is not tested for tetracycline, so we cannot guarantee that the product is tetracycline free. Product F2442 is regularly tested for tetracycline and the results shown in a lot-specific certificate of analysis. The website page for this product is linked here: https://www.sigmaaldrich.com/catalog/product/sigma/f2442?lang=fr®ion=CH
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When aliquioting FBS, is it best to cool them to 4ºC first or directly at -80ºC
1 answer-
The serum can be aliquoted into smaller, single-use portions and refrozen directly.
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Hello, I have a question regarding FBS (F7524), for my experiment it is essential there is no dopamine or serotonine in my medium. Is it known wether these substances are present in FBS?
1 answer-
This product has not undergone testing to determine the presence of dopamine or serotonin.
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Could the presence of insoluble precipitates when thawing the serum indicate contamination?
1 answer-
Thawed serum may contain precipitates and flocculants due to various reasons, such as residual fibrinogen converting to fibrin upon thawing. Centrifugation at 400 xg can remove excess fibrin, while filtering serum is not recommended to avoid protein depletion and clogged filters.
Additional points to consider:
•Cloudiness in human serum products is common and indicates the presence of lipids. Clear, lipid-free human serum products are not available.
•Microscopic serum precipitates may resemble yeast contamination but are actually lipid aggregates that can be dissolved by lipase. They do not show growth in culture or gram staining and can be removed through centrifugation or filtration.
•Gel layer or clumps in serum are caused by poor or overheated inactivation or improper mixing of FBS during the Heat inactivation process. Serum with these characteristics should not be used.
•To minimize denaturation of serum lipoproteins and turbidity, it is recommended to prepare single-use aliquots and avoid repeated freeze-thaw cycles.Helpful?
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Is this product F7524 heat-inactivated? Should it be prior to use?
1 answer-
This product has not been heat inactivated. For most cell culture applications heat inactivation is not necessary. If the application requires inactivation of any heat labile serum components, such as compliment or cytokines, the product may be heat inactivated in the lab. Alternatively, a heat inactivated version of this product is also available. See product F9665.
To heat inactivate this product:
1. Allow serum containers to come to room temperature. Swirl bottles of serum immediately before adding to the water bath.
2. A water-filled control bottles (T1) should be placed in a 56 °C water bath. Place the containers in the water bath up to the serum line. Do not completely submerge the containers. When the temperature of T1 reaches 56 °C, start the timer set for 30 minutes. Do not allow temperatures to exceed 56 °C. Higher temperatures will denature the proteins, gel the serum or alter the performance of the serum.
3. Gently swirl the bottles every 5 to 10 minutes while in the water bath and check the temperature in the control bottle. It is not uncommon for plastic containers to bow slightly after heat treatment. This is not harmful to the serum.
4. At the end of 30 minutes remove the serum bottles from the water bath. Aliquot the serum into smaller volumes and freeze. Avoid repeated thaw-freeze cycles.Helpful?
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Bonjour, Quelle est la composition et la concentration du sérum référencé F7524 en Calcium ? Cordialement
1 answer-
Calcium levels are not determined for this product. The serum is a natural product that will contain calcium at physiological levels.
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Welches fetale Kälbserum wurde für die Kryokonservierung von Mausembryonen oder Eiestockmäus verwendet?
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Unfortunately, a fetal calf serum that has been specifically tested for cryopreservation of mouse embryos or ovarian mice is not currently offered. Please see the link below to review Product ES009-M, Fetal Bovine Serum. This product is tested for expansion and cytotoxicity of mouse embryonic stem cells and may be of interest:
https://www.sigmaaldrich.com/product/mm/es009mHelpful?
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How can we heat inactivate the fetal bovine serum cat: F7524? For how long?
1 answer-
All heat inactivated serum products are treated at 56 deg. C for 30 minutes. This process may be applied to manually heat inactivate serum on a per bottle basis using a water bath. When immersing bottles do not allow water to come in contact with the cap/closure.
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