Yes. It is Ficoll 400. This product is graded molecular biology grade and is free of any nucleases
F2637
Polysucrose 400
BioXtra, for molecular biology, lyophilized powder
Synonyme(s) :
Polysucrose 400, Ficoll 400
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About This Item
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Source biologique
non-animal source
Niveau de qualité
Qualité
Molecular Biology
for molecular biology
Gamme de produits
BioXtra
Forme
lyophilized powder
Activité étrangère
DNase (Exonuclease), NICKase (Endonuclease), RNase and Protease, none detected
Température de stockage
room temp
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Description générale
Ficoll® 400 is a non-ionic synthetic polymer of sucrose used for cell separation and organ isolation.
Application
Utilisé pour la préparation des cellules et l′isolement des organelles
Suitable for
- hybridization experiments to reduce non-specific binding of labeled probes to the membrane[1]
- preparation of DNA loading buffer (15% Ficoll®, 0.25% bromophenol blue and 0.25% xylene cyanol FF
- separation of cells with similar density but different size (PBMC from human peripheral blood, bone marrow and umbilical cord blood)
- producing density gradients to separate subcellular components
- vitrification of cell and tissue samples.[2]
- to compare the effect of different perfusates on Hypoxic pulmonary vasoconstriction (HPV).[3]
Caractéristiques et avantages
- free from DNases, RNases, phosphatases and proteases
- preserves functional and morphological integrity of the separated cells
Autres remarques
Un polymère synthétique non ionique du sucrose
Informations légales
Ficoll is a registered trademark of Cytiva
Produit(s) apparenté(s)
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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Lot/Batch Number
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Protocoles
Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.
Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.
Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.
Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.
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Is Product F2637, Ficoll, the same as Ficoll 400?
1 answer-
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What is the difference between Ficoll 400 and Ficoll PM 400?
1 answer-
The difference between Ficoll 400 and Ficoll PM 400 is the PM 400 has stricter specifications on the number of microorganisms/g and the endotoxin level is < 0.125 EU/ml
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Do you have a protocols for use of Product F2637, Ficoll, in various applications?
1 answer-
Please check the product information sheet for F4375 which has a detailed protocol.
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Can you use Product F2637, Ficoll, to isolate mononuclear cells?
1 answer-
When trying to isolate mononuclear cells you can use this product but we offer Histopaque that is a mixture of Ficoll and Hypaque. For humans, we offer H8889, Histopaque®-1077 Hybri-Max™ or 1077-1, Histopaque®-1077. For rodents we offer 1083-1, Histopaque®-1083. For isolation of neutrophils, we offer 1119-1, Histopaque®-1119.
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Can Product F2637, Ficoll, be used as a component of a DNA loading buffer?
1 answer-
This product can be used in a DNA loading buffer with the following recipe to prepare a 6X stock:0.25% bromophenol blue0.25% xylene cyanol FF15% Ficoll
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