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B2434

Sigma-Aldrich

Sulfate de bléomycine from Streptomyces verticillus

Bleomycin sulfate from Streptomyces verticillus
1 of 1 reviewers received a sample product or took part in a promotion

BioXtra, crystalline

Synonyme(s) :

Blexane, Blénoxane, Bléo

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About This Item

Numéro CAS:
9041-93-4
Numéro CE :
232-925-2
Numéro MDL:
MFCD00070310
Code UNSPSC :
51111733
ID de substance PubChem :
24891650
Nomenclature NACRES :
NA.85
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Gamme de produits

BioXtra

Niveau de qualité

200

Forme

crystalline

Puissance

1.5-2.0 units per mg

Technique(s)

cell culture | mammalian: suitable

Solubilité

H2O: 20 mg/mL, clear, colorless

Traces de cations

Cu: ≤0.10%

Spectre d'activité de l'antibiotique

Gram-negative bacteria
Gram-positive bacteria
neoplastics

Mode d’action

DNA synthesis | interferes

Température de stockage

2-8°C

Chaîne SMILES 

[O-]S([O-])(=O)=O.C[C@@H](O)[C@@H](NC(=O)[C@@H](C)[C@H](O)[C@@H](C)NC(=O)[C@H](NC(=O)c1nc(nc(N)c1C)[C@H](CC(N)=O)NC[C@H](N)C(N)=O)[C@@H](O[C@@H]2O[C@@H](CO)[C@@H](O)[C@H](O)[C@@H]2O[C@@H]3O[C@H](CO)[C@@H](O)[C@@H](OC(N)=O)[C@@H]3O)c4c[nH]cn4)C(=O)NCCc5nc(cs5)-c6ncc(s6)C(=O)NCCC[S+](C)C.C[C@@H](O)[C@@H](NC(=O)[C@@H](C)[C@H](O)[C@@H](C)NC(=O)[C@H](NC(=O)c7nc(nc(N)c7C)[C@H](CC(N)=O)NC[C@H](N)C(N)=O)[C@@H](O[C@@H]8O[C@@H](CO)[C@@H](O)[C@H](O)[C@@H]8O[C@@H]9O[C@H](CO)[C@@H](O)[C@@H](OC(N)=O)[C@@H]9O)c%10c[nH]cn%10)C(=O)NCCc%11nc(cs%11)-c%12ncc(s%12)C(=O)NCCC[S+](C)C

InChI

1S/2C55H83N17O21S3.H2O4S/c2*1-20-33(69-46(72-44(20)58)25(12-31(57)76)64-13-24(56)45(59)82)50(86)71-35(41(26-14-61-19-66-26)91-54-43(39(80)37(78)28(16-73)90-54)92-53-40(81)42(93-55(60)88)38(79)29(17-74)89-53)51(87)67-22(3)36(77)21(2)47(83)70-34(23(4)75)49(85)63-10-8-32-68-27(18-94-32)52-65-15-30(95-52)48(84)62-9-7-11-96(5)6;1-5(2,3)4/h2*14-15,18-19,21-25,28-29,34-43,53-54,64,73-75,77-81H,7-13,16-17,56H2,1-6H3,(H13-,57,58,59,60,61,62,63,66,67,69,70,71,72,76,82,83,84,85,86,87,88);(H2,1,2,3,4)/t2*21-,22+,23+,24-,25-,28-,29+,34+,35+,36-,37+,38+,39-,40-,41-,42+,43-,53-,54-;/m00./s1

Clé InChI

OOXTWFJZZAJGKA-CNLAFNBISA-N

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Catégories apparentées

Description générale

Chemical structure: glycopeptide

Application

Bleomycin Sulfate is an antineoplastic antibiotic isolated from Streptomyces verticillus. It is recommended for use as a cell culture agent at 10-100 μg/mL.

Actions biochimiques/physiologiques

Le sulfate de bléomycine se lie à l′ADN, provoque une coupure de l′ADN double brin au niveau de séquences de bases spécifiques et inhibe la synthèse de l′ADN. Cette action inhibitrice nécessite la liaison d′oxygène et d′un ion métallique par la bléomycine. Elle peut également cliver l′ARN dans une moindre mesure, mais de façon plus sélective. Elle agit comme inducteur et régulateur de l′apoptose et inhibe l′angiogenèse tumorale.

Composants

Ce produit est un mélange d′antibiotiques glycopeptidiques contenant les bléomycines A2 (70 %) et B2 (30 %). Les bléomycines se distinguent les unes des autres par leur amine terminale et leur activité biologique.

Attention

Il est recommandé de conserver ce produit entre 2 et 8 °C.

Notes préparatoires

Ce produit est soluble dans l′eau à 20 mg/ml et maintient son activité pendant plusieurs jours. Une solution à 2 unités/ml dans du phosphate de potassium neutre à 0,1 M doit être utilisée dans un délai de 14 jours. Une solution à 3 unités/ml dans un tampon salin normal reste active pendant trois mois.

Autres remarques

Keep container tightly closed in a dry and well-ventilated place, strongly hygroscopic

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H340,H351,H361fd

Classification des risques

Carc. 2 - Muta. 1B - Repr. 2

Code de la classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges

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Certificats d'analyse (COA)

Lot/Batch Number
0000313599
0000313599
0000276411
0000276411
SLCM6973

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Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Z G Fridlender et al.
The European respiratory journal, 30(2), 205-213 (2007-05-17)
Epithelial cell injury and apoptosis are recognised as early features in idiopathic pulmonary fibrosis and bleomycin-induced fibrosis in mice. Telomerase is a known apoptosis-alleviating factor. The role of telomerase was studied during bleomycin-induced lung epithelial cell (LEC) apoptosis in vitro
Abdulaleem Alnajar et al.
PloS one, 8(11), e81356-e81356 (2013-11-22)
Fibrogenesis is usually initiated when regenerative processes have failed and/or chronic inflammation occurs. It is characterised by the activation of tissue fibroblasts and dysregulated synthesis of extracellular matrix proteins. FHL2 (four-and-a-half LIM domain protein 2) is a scaffolding protein that
Andrea M Foskett et al.
American journal of physiology. Lung cellular and molecular physiology, 306(2), L120-L131 (2013-11-19)
Previous reports demonstrated that bleomycin-induced injury of lungs in mice can be improved by the administration of murine multipotent adult stem/progenitor cells (MSCs) from the bone marrow. Recently some of the beneficial effects of MSCs have been explained by the
Alexander M Cameron et al.
Plastic and reconstructive surgery, 133(1), 69-78 (2014-01-01)
The development of new therapies for hypertrophic scarring has been hampered by the lack of an appropriate animal model. The authors' objective was to establish a reproducible murine model of hypertrophic scarring by infusing bleomycin over a prolonged period to
Britta Maurer et al.
Annals of the rheumatic diseases, 72(12), 2039-2046 (2013-09-10)
To assess whether the discrepancy between the strong antifibrotic effects of tyrosine kinase inhibitors (TKIs) in animal models and the inconsistent results in clinical studies might be related to the activation levels of drug targets. Skin sections of bleomycin, TSK1
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Articles

DNA Damage and Repair

DNA damage and repair mechanism is vital for maintaining DNA integrity. Damage to cellular DNA is involved in mutagenesis, the development of cancer among others.

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Questions

1–2 of 2 Questions  

  1. What is the unit/mg for this product?

    1 answer

    1. This product has a potency specification of 1.5-2.0 units per mg. The exact value will be listed on the lot specific Certificate of Analysis. See the link below to review a sample Certificate of Analysis: https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/B2434-BULKSLCM6973.pdf

      Helpful?

  2. I can't find an expiration or use by date for the powder. Is it better to resuspend and freeze upon arrival or is it better to keep at 4deg until ready to resuspend and freeze?

    1 answer

    1. This product does not contain an expiration date or recommended retest date in its crystalline format. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment.

      This product should be placed in the proper storage temperature of 2-8°C upon arrival. Once reconstituted, the material should be stored at 2-8°C as well. For reconstitution instructions and stability, please see "Preparation Notes" section of the product page.

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Reviews

1 of 1 reviewers received a sample product or took part in a promotion

Active Filters

  1. Boston, MA
    • Review 1
    • Votes 0
    3 out of 5 stars.

    Needs to optimize product packaging (container)

    Could be beneficial if the vial is smaller or the product is in liquid form. The quantity is too little with respect to the size of the container, also the product (powder) sticks to the container

    Helpful?

    1. Response from MilliporeSigma:

      Thank you for taking the time to leave a review! We appreciate your feedback and have passed on your concerns to our Product Management teams. Please do not hesitate to contact us at https://www.sigmaaldrich.com/support/customer-support and submit a Product Technical Inquiry for further assistance.

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