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Merck
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Key Documents

ES009

Millipore

p-NPP Single Reagent, Yellow, Alkaline Phosphatase Substrate (soluble)

500 ml p-NPP Single Reagent, Yellow, Alkaline Phosphatase Substrate (soluble) for ELISA.

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About This Item

Code UNSPSC :
41116010
eCl@ss :
42029053
Nomenclature NACRES :
NB.22

Forme

liquid

Fabricant/nom de marque

Chemicon®

Technique(s)

ELISA: suitable

λmax

405 nm

Application

p-Nitrophenyl phosphate (p-NPP) is the customary product utilized for the assay of alkaline phosphatase. It is usually supplied in tablet or powder form and is dissolved in an appropriate buffer immediately prior to assay. Liquid p-NPP concentrates are also available, but must also be diluted in buffer. CHEMICON, Inc. now provides p-NPP stabilized in diethanolamine (DEA) buffer and available for instant use. The DEA buffer contains Mg++ and Zn++ ions providing optimum activation of alkaline phosphatase labeled probes.

This is an excellent substrate for phosphatase based ELISA assays.

It produces a soluble end product which is yellow in color and reads at 405 nm to 410 nm. Both kinetic and end point determinations can be performed.

Forme physique

Color Code: Yellow
p-NPP, a single component substrate in proprietary stabilizer.

Stockage et stabilité

Maintain at 2-8°C for up to one year.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictogrammes

Health hazardCorrosion
GHS08,GHS05

Mention d'avertissement

Danger

Mentions de danger

H315,H318,H361fd

Classification des risques

Eye Dam. 1 - Repr. 2 - Skin Irrit. 2

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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