N7653

p-Nitrophenyl Phosphate Liquid Substrate System

alkaline phosphatase substrate, liquid

• Synonyme(s) : 4-Nitrophenyl phosphate disodium salt solution, pNPP
• Formule linéaire : O₂NC₆H₄OP(O)(ONa)₂
• Numéro CAS: 4264-83-9
• Poids moléculaire : 263.05
• UNSPSC Code: 12352204
• PubChem Substance ID: 24897835
• NACRES: NA.83
• MDL number: MFCD00007319

Propriétés

• Nom du produit: p-Nitrophenyl Phosphate Liquid Substrate System, liquid
• form: liquid
• Quality Segment: 200
• shipped in: wet ice
• storage temp.: −20°C
• SMILES string: [Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
• InChI: 1S/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2
• InChI key: VIYFPAMJCJLZKD-UHFFFAOYSA-L

Description

General description

The p-Nitrophenyl Phosphate (pNPP) Liquid Substrate System combines p-nitrophenyl phosphate, buffer, and the required magnesium cations in a convenient, ready-to-use, single solution reagent. Producing a soluble end product, pNPP is the substrate of choice in alkaline phosphatase enzyme immunoassays.

Application

p-Nitrophenyl Phosphate Liquid Substrate System has been used as a substrate to determine the activity of alkaline phosphatase in alkaline phosphatase assay.

Substrate for alkaline phosphatase that yields a soluble yellow end product that may be read at 405 nm. The reaction may be stopped with 3N NaOH and read at 405 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.

Biochem/physiol Actions

4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP produces p-nitrophenol. It is a common chromogenic substrate for alkaline phosphatase.

Physical form

Ready-to-use.

Disclaimer

Stable at least one year when stored below 0 °C.

Informations sur la sécurité

• pictograms: GHS08,GHS05
• signalword: Danger
• hcodes: H290,H315,H318,H361fd,H373
• pcodes: P202 - P234 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Eye Dam. 1 - Met. Corr. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral
• target_organs: Kidney,Liver,Blood
• Classe de stockage: 12 - Non Combustible Liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable