SRP6185

Factor Va from human plasma

≥95% (SDS-PAGE)

• Synonyme(s) : Activated protein C cofactor, Proaccelerin (labile factor)
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human plasma
• Pureté: ≥95% (SDS-PAGE)
• Forme: liquid
• Poids mol.: heavy chain 94.0 kDa; light chain 74 kDa
• Conditionnement: pkg of 10 μg
• Numéro d'accès UniProt: P12259
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Informations sur le gène: human ... F5(2153)

Description

Description générale

Factor Va is a cofactor for the serine protease factor Xa, and in the presence of calcium ions they collectively assemble on a phospholipid surface to form the prothrombinase complex. The prothrombinase complex is responsible for the rapid conversion of prothrombin to thrombin. Factor Va is derived from the pro-cofactor, factor V, upon limited proteolysis by α-thrombin. The thrombin cleavage of factor V liberates two heavily glycosylated activation peptides from the central portion of the molecule which have no cofactor function. Factor Va is comprised of an NH2-terminal derived heavy chain (Mr=94,000) and a COOH-terminal derived light chain (Mr=74,000) which remain associated in the presence of calcium ions. The cofactor binds to phospholipid (cell membrane) surfaces and effectively serves as a receptor for membrane bound factor Xa. Complete assembly of the prothrombinase complex (factor Xa, factor Va, phospholipid, and calcium) results in a 300,000-fold increase in the rate of prothrombin conversion relative to the rate observed with factor Xa alone. The interaction between factor Va and factor Xa is mediated by both the heavy and light chain of factor Va, while the binding of prothrombin to factor Va is mediated solely by the heavy chain.

Forme physique

liquid in 41.3 μl 20 mM HEPES, 150 mM NaC1, pH 7.5, 5 mM CaCl2 +50% glycerol.

Informations sur la sécurité

• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable