P6486
EZview™ Red Protein A Affinity Gel
Synonyme(s) :
EZview Affinity Gel, Protein A Affinity Gel
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1 ML
319,00 $
5 X 1 ML
1 240,00 $
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1 ML
319,00 $
5 X 1 ML
1 240,00 $
About This Item
319,00 $
Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
Produits recommandés
Forme
liquid
Classe(s) chimique(s) de l'analyte
proteins (Immunoglobulins of various mammalian species)
Technique(s)
affinity chromatography: suitable
immunoprecipitation (IP): suitable
Capacité
~4 mg binding capacity (rabbit IgG)
Conditions d'expédition
wet ice
Température de stockage
−20°C
Catégories apparentées
Description générale
EZview™ Red Protein A Affinity Gel is a highly visible, red colored Protein A affinity gel. The affinity resin contains Staphylococcus aureus Protein A covalently attached to crosslinked 4% agarose beads. The affinity gel is used to bind the FC portion of the IgG of an antibody to samples, such as cell lysates and tissue. The immune complexes, bound to the EZview™ Red Protein A Affinity Gel, are recovered by centrifugation. Red dye enhances visibility for more efficient results.
Application
EZview™ Red Protein A Affinity Gel has been used for purification of proteins in the preparation of immunoprecipitation[1][2] and chromatin immunoprecipitation (ChIP)[3] assays.
When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduce the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.
Caractéristiques et avantages
- Increased visibility - Red color reduces risk of incidental aspiration
- Improved recovery of target protein by reduced accidental loss
- Higher reproducibility - More consistent yields
Forme physique
1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon
50% slurry in PBS with 50%Glycerol and 0.0015%
Stockage et stabilité
EZview™ Red Protein A Affinity Gel should be stored in 50% glycerol at -20 °C. This product is stable for at least one year when stored as indicated. Do not freeze in the absence of glycerol.
Informations légales
EZview is a trademark of Sigma-Aldrich Co. LLC
Clause de non-responsabilité
For U.S. Customers: Contains mercury; Do not place in trash - dispose according to local, state, or federal laws.
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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