P4557

Phénol solution

BioReagent, Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, for molecular biology

• Synonyme(s) : Tris-equilabrated Phenol
• Formule empirique (notation de Hill): C₆H₆O
• Numéro CAS: 108-95-2
• Poids moléculaire : 94.11
• Beilstein: 6428463
• Numéro CE : 203-632-7
• Numéro MDL: MFCD00002143
• Code UNSPSC : 12352104
• ID de substance PubChem : 57654529
• Nomenclature NACRES : NA.52
• Qualité: for molecular biology
• Essai: ≥99.0% (GC)

Propriétés

• Qualité: for molecular biology
• Niveau de qualité: 200
• Gamme de produits: BioReagent
• Essai: ≥99.0% (GC)
• Forme: liquid
• pH: 7.7-8.1 (25 °C)
• Température de stockage: 2-8°C
• Chaîne SMILES: Oc1ccccc1
• InChI: 1S/C6H6O/c7-6-4-2-1-3-5-6/h1-5,7H
• Clé InChI: ISWSIDIOOBJBQZ-UHFFFAOYSA-N
• Informations sur le gène: human ... GABRA1(2554)

Description

Description générale

A tris-equilibrated phenol solution is a clear liquid capped with a layer of Tris buffer. Phenol solutions can be used for a wide variety of molecular biology applications. Organic compounds like phenols have an aromatic carbocyclic ring with a hydroxyl group attached directly to it. The common name for monohydroxybenzene is phenol.

Application

Phenol solution has been used:

• as a component of the digestion reaction mix to prepare RNase-free plasmids for in vitro translation in a rabbit reticulocyte lysate
• for extraction of DNA from Arabidopsis thaliana Rosette leaves
• in the extraction of DNA from blood on preservation paper by standard phenol-chloroform protocol
• as a component of extraction buffer to extract RNA from fruit pulp
• for the extraction of protein from roots
• for the isolation of fecal DNA
• for the extraction of caged RNA

Actions biochimiques/physiologiques

The phenol-chloroform extraction method is widely employed to extract nucleic acids. Even though phenol does not completely inhibit RNase activity, its unique characteristic makes it beneficial for the isolation of nucleic acids when used in combination with chloroform and alcohol.

Composants

• 1 bottle Tris-equilabrated Phenol (product number P4557)
• Equilibration Buffer (product B5658)

Informations sur la sécurité

• Pictogrammes: GHS06,GHS08,GHS05,GHS09
• Mention d'avertissement: Danger
• Mentions de danger: H301 + H311 + H331,H314,H341,H373,H411
• Conseils de prudence: P202 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Classification des risques: Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
• Organes cibles: Nervous system,Kidney,Liver,Skin
• Code de la classe de stockage: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter