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P4505

Sigma-Aldrich

Protamine sulfate

powder

Synonyme(s) :

Clupeine

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About This Item

Numéro CAS:
9007-31-2
Numéro MDL:
MFCD00132091
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.77

product name

Protamine sulfate salt from herring, Grade III, histone, free(Millon test)

Source biologique

fish (herring)

Type

Grade III

Forme

powder

Impuretés

histone, free (Millon test)

Solubilité

6 M HCl: 25 mg/mL, clear, colorless to very faintly yellow

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Application

May be used for the removal of DNA from protein samples or for the purification of DNA binding proteins. It may be used as an alternative to polybrene in retroviral-mediated gene transfer.

Actions biochimiques/physiologiques

Protamine in the form of solid lipid nanoparticles (SLN) promoted transfection with plasmid DNA more efficiently and with less cytotoxicity than comparable SLNs composed of Esterquat-1.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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Moyra Lawrence et al.
Science advances, 8(7), eabj8618-eabj8618 (2022-02-17)
Platelet deficiency, known as thrombocytopenia, can cause hemorrhage and is treated with platelet transfusions. We developed a system for the production of platelet precursor cells, megakaryocytes, from pluripotent stem cells. These cultures can be maintained for >100 days, implying culture
A Bonincontro et al.
Physics in medicine and biology, 34(5), 609-616 (1989-05-01)
The complex dielectric constant of arginine and protamine from herring sperm (clupeine) and their complexes with herring sperm DNA was measured at 10 GHz in the temperature range -20 to +45 degrees C by a microwave cavity perturbation method. The
L Willmitzer et al.
Biophysics of structure and mechanism, 6(2), 95-110 (1980-01-01)
The thermodynamics of protamine-DNA interation was investigated with clupeine Z from herring labeled at its amino terminus with fluorescein. The ionic strength dependence, the influence of protamine phosphorylation, of the native DNA conformation, using native and heat-denatured DNA, and of
Chengming Liu et al.
BMC medicine, 20(1), 187-187 (2022-05-14)
Cytokines have been reported to alter the response to immune checkpoint inhibitors (ICIs) in patients with the tumor in accordance with their plasma concentrations. Here, we aimed to identify the key cytokines which influenced the responses and stimulated resistance to
L T Hansen et al.
International journal of food microbiology, 66(3), 149-161 (2001-06-29)
The antimicrobial effect of protamine (clupeine) on a range of gram-positive and gram-negative foodborne pathogens and spoilage bacteria, was evaluated using an agar dilution assay and a broth dilution assay with Alamar Blue as growth indicator. Protamine was tested alone
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