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M8537

Sigma-Aldrich

MCDB 131 Medium

With ʟ-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonyme(s) :

131 Medium, Medium 131, Molecular, Cellular, and Development Biology 131 medium

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75

product name

MCDB 131 Medium, With trace elements and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Forme

powder

Concentration

11.6 g/L

Technique(s)

cell culture | mammalian: suitable

Composants

sodium pyruvate: 0.11 g/L
phenol red: 0.012 g/L
L-glutamine: 1.461 g/L
HEPES: no
glucose: 1.0 g/L (Dextro)

Conditions d'expédition

ambient

Température de stockage

2-8°C

Catégories apparentées

Description générale

MCDB-131 medium provides a defined and optimally balanced nutritional environment that selectively promotes the growth of specific cell types. MCDB-131 is designed to support clonal growth of human microvascular endothelial cells, including human umbilical vein endothelial cells, when supplemented with 0.7% FBSP, 10 ng/ml epidermal growth factor, and 1 μg/ml hydrocortisone. MCDB media is made for the low-protein or serum-free growth of specific cell types using hormones, growth factors, trace elements, or low levels of dialyzed fetal bovine serum protein (FBSP). 

Application

MCDB-131 Medium has been used:

  • as a component of the basal medium to culture bovine retinal endothelial cells
  • to culture bovine lung microvascular endothelial cells
  • for the isolation of porcine endocranial endothelial cells and porcine aortic endothelial cells

Quantité

Formulated to contain 11.6 grams of powder per liter of medium.

Reconstitution

Supplement with 1.18 g/L sodium bicarbonate.

Souvent commandé avec ce produit

Réf. du produit
Description
Tarif

Supplément

Réf. du produit
Description
Tarif

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Abdul Jaleel et al.
Molecular and cellular biochemistry, 451(1-2), 1-10 (2018-06-24)
Endocardial endothelium, which lines the chambers of the heart, is distinct in its origin, structure, and function. Characterization studies using genomics and proteomics have reported molecular signatures supporting the structural and functional heterogeneity of various endothelial cells. However, though functionally
Kathleen M Job et al.
Anesthesia and analgesia, 123(2), 382-393 (2016-06-23)
The endothelial glycocalyx is an important component of the vascular permeability barrier, forming a scaffold that allows serum proteins to create a gel-like layer on the endothelial surface and transmitting mechanosensing and mechanotransduction information that influences permeability. During acute inflammation
Thomas P Burke et al.
eLife, 10 (2021-08-24)
Arthropod-borne rickettsial pathogens cause mild and severe human disease worldwide. The tick-borne pathogen Rickettsia parkeri elicits skin lesions (eschars) and disseminated disease in humans; however, inbred mice are generally resistant to infection. We report that intradermal infection of mice lacking
Sandra S Hammer et al.
Diabetologia, 64(7), 1674-1689 (2021-03-27)
Homo sapiens evolved under conditions of intermittent food availability and prolonged fasting between meals. Periods of fasting are important for recovery from meal-induced oxidative and metabolic stress, and tissue repair. Constant high energy-density food availability in present-day society contributes to
G S Ajithkumar et al.
Cardiovascular toxicology, 16(4), 390-405 (2015-12-15)
Endocardial endothelial cells (EECs), when compared with endothelial cells of arteries and veins, possess higher resistance to apoptosis-inducing anticancer agents. The mechanism of this resistance property is unknown. We have investigated the molecular mechanism, which contributes to increased cell survival
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Protocoles

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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