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L9776

Sigma-Aldrich

Leucine Aminopeptidase, microsomal from porcine kidney

suitable for protein sequencing, BioReagent, lyophilized powder

Synonyme(s) :

Aminopeptidase M, Aminopeptidase N, Cathepsin III, LAP, Leucinamide aminopeptidase, Leucinaminopeptidase

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About This Item

Numéro CAS:
9054-63-1
Numéro de classification (Commission des enzymes):
3.4.11.2 (BRENDA, IUBMB)
Numéro CE :
232-942-5
Numéro MDL:
MFCD00131501
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.26

Gamme de produits

BioReagent

Forme

lyophilized powder

Conditionnement

vial of 50 μg

Adéquation

suitable for protein sequencing

Application(s)

metabolomics
vitamins, nutraceuticals, and natural products

Température de stockage

−20°C

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Description générale

Leucine Aminopeptidase (LAP) is a proteolytic enzyme. It is an amino acid hydrolase that catalyzes the hydrolysis of leucine residues from the amino-terminal of proteins or peptides. LAP is naturally present in the liver, kidney, lung, and serum.

Application

Leucine Aminopeptidase has been used:
  • in proteomics analysis
  • as a treatment for protein depolymerization reaction
  • to test its effect on the degradation of human recombinant IL-8

Actions biochimiques/physiologiques

Leucine Aminopeptidase serves as a biomarker for drug-induced liver injury because in case of liver injury, cirrhosis, hepatitis, and other disorders serum LAP activity increases. Abnormal expression of LAP results in tumor cell proliferation, angiogenesis, and invasion, so it can also serve as a cancer-related biomarker.

Définition de l'unité

One unit will hydrolyze 1.0 μmole of L-leucine-p-nitroanilide to L-leucine and p-nitroaniline per min at pH 7.2 at 37 °C. (At 25 °C, approx. 40% of the activity at 37 °C is obtained.) The activity obtained using L-leucine p-nitroanilide as substrate is 2-5 times that obtained with L-leucinamide as substrate.

Inhibiteur

Réf. du produit
Description
Tarif

B8385

Bestatin hydrochloride, ≥98% (HPLC)

A6671

Actinonin

A1276

Amastatin hydrochloride hydrate, ≥97% (HPLC)

Pictogrammes

Health hazardExclamation mark
GHS08,GHS07

Mention d'avertissement

Danger

Mentions de danger

H315,H319,H334,H335

Classification des risques

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Simone Giaveri et al.
Advanced materials (Deerfield Beach, Fla.), 33(44), e2104581-e2104581 (2021-09-24)
The billion tons of synthetic-polymer-based materials (i.e. plastics) produced yearly are a great challenge for humanity. Nature produces even more natural polymers, yet they are sustainable. Proteins are sequence-defined natural polymers that are constantly recycled when living systems feed. Digestion
A Kehlen et al.
European cytokine network, 12(2), 316-324 (2001-06-12)
In rheumatic joints, high concentrations of interleukin-8 (IL-8) have been measured in synovial fluid and in pannus tissue. In both locations aminopeptidase N (APN)/CD13, an exopeptidase with reported activity towards IL-8 is also present. The surprising stability of IL-8 in
Yong Huang et al.
Analytical chemistry, 91(13), 8085-8092 (2019-05-31)
Drug-induced liver injury (DILI) is a frequent cause of hepatic dysfunction as well as the single most frequent reason for removing approved medications from the market, and multispectral optoacoustic tomography (MSOT) is an emerging and noninvasive imaging modality for diagnosing
Fluorescent probes for detection and bioimaging of leucine aminopeptidase
Y Chen
Materials today chemistry, 15, 100216-100216 (2020)
Juan Reguera et al.
PLoS pathogens, 8(8), e1002859-e1002859 (2012-08-10)
The coronaviruses (CoVs) are enveloped viruses of animals and humans associated mostly with enteric and respiratory diseases, such as the severe acute respiratory syndrome and 10-20% of all common colds. A subset of CoVs uses the cell surface aminopeptidase N
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