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GUSS

Sigma-Aldrich

β-Glucuronidase Reporter Gene Staining Kit

Synonyme(s) :

β-Glucuronidase Staining Kit, Reporter Gene Kit, Staining Kit for Glucuronidase

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.32

Utilisation

 kit sufficient for 100 histochemical assays

Niveau de qualité

200

Technique(s)

microbe id | staining: suitable

Conditions d'expédition

dry ice

Température de stockage

−20°C

Description générale

Kit is used to anaylze β glucuronidase (E.coli GUS gene) expression in transformed plants.

Application

The E. coli GUS gene is extensively used to analyze gene expression in transformed plants. Plants have low intrinsic GUS activity, and the E. coli gene is quite stable in plant cells. As a tag, GUS remains active at the N-terminus of fusion proteins.

Kit is suitable for biochemical activity assays, immunological assays and histochemical staining of tissue and cells.

Caractéristiques et avantages

  • Histochemical staining of plant tissues expressing the E. coli GUS enzyme
  • Ideally suited to plant expression studies due to very low GUS activity in plants and high enzyme stability
  • GUS does not interfere with plant cell function or viability

Autres remarques

The substrate used in this kit is X-GlcA (5-bromo-4-chloro-3-indolyl β-D-glucuronide) which results in an insoluble indigo-blue precipitate in transfected cells and tissues.

Composants de kit seuls

Réf. du produit
Description
  • 2× Fixation Buffer 25 mL
  • 5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt, ≥98% 1 mL/vial
  • Reagent A for gus staining kit 50 mL
  • Reagent B for gus staining kit 200 μL
  • Reagent C for gus staining kit 200 μL

Mentions de danger

H412

Conseils de prudence

P273 - P501

Classification des risques

Aquatic Chronic 3

Code de la classe de stockage

11 - Combustible Solids

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

C-M Cheng et al.
Cancer gene therapy, 15(6), 393-401 (2008-03-29)
Increasing the specificity of chemotherapy may improve the efficacy of cancer treatment. Toward this aim, we developed a strain of bacteria to express enzymes for selective prodrug activation and non-invasive imaging in tumors. beta-glucuronidase and the luxCDABE gene cluster were
Li-Ling Lin et al.
International journal of molecular sciences, 14(7), 14270-14286 (2013-07-11)
Brassinosteroids (BRs) are endogenous plant hormones and are essential for normal plant growth and development. MicroRNAs (miRNAs) of Arabidopsis thaliana are involved in mediating cell proliferation in leaves, stress tolerance, and root development. The specifics of BR mechanisms involving miRNAs
Kuo-Hsiang Chuang et al.
Bioconjugate chemistry, 17(3), 707-714 (2006-05-18)
Combination therapy can help overcome limitations in the treatment of heterogeneous tumors. In the current study, we examined whether multiple therapeutic agents could be targeted to anti-dansyl single-chain antibodies (DNS scFv) that were anchored on the plasma membrane of cancer
R A Jefferson et al.
The EMBO journal, 6(13), 3901-3907 (1987-12-20)
We have used the Escherichia coli beta-glucuronidase gene (GUS) as a gene fusion marker for analysis of gene expression in transformed plants. Higher plants tested lack intrinsic beta-glucuronidase activity, thus enhancing the sensitivity with which measurements can be made. We
R A Jefferson
Nature, 342(6251), 837-838 (1989-12-14)
The GUS reporter gene system is already a powerful tool for the assessment of gene activity in transgenic plants. Further developments may lead to routine in vivo analysis and fusion genetics.
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