E1510

Ethidium bromide solution

BioReagent, for molecular biology, 10 mg/mL in H₂O

• Synonyme(s) : 3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide, EtBr, Homidium bromide
• Formule empirique (notation de Hill): C₂₁H₂₀BrN₃
• Numéro CAS: 1239-45-8
• Poids moléculaire : 394.31
• Numéro Beilstein : 3642536
• Numéro MDL: MFCD00011724
• Code UNSPSC : 41105322
• ID de substance PubChem : 24894418
• Nomenclature NACRES : NA.31

Propriétés

• Qualité: for molecular biology
• Niveau de qualité: 200
• Gamme de produits: BioReagent
• Concentration: 10 mg/mL in H₂O
• Technique(s): electrophoresis: suitable
• Adéquation: suitable for gel electrophoresis
• Chaîne SMILES: [Br-].CC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14
• InChI: 1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H
• Clé InChI: ZMMJGEGLRURXTF-UHFFFAOYSA-N

Description

Application

Ethidium bromide solution has been used:

• as a stain to visualize U937 cells to assess cell viability
• to detect polymerase chain reaction products
• in agarose gel electrophoresis based gel retardation assay

Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.

Actions biochimiques/physiologiques

Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.

Reconstitution

For staining a gel after electrophoresis, dilute a sample of the stock solution to 0.5 μg/ml with water and incubate the gel for 15-30 min. Destaining is usually not needed but can be carried out in water for 15 min if decreased background is necessary. The DNA bands can then be detected on a UV light box (254 nm wavelength). Ethidium bromide can also be incorporated into the gel and running buffer at 0.5 μg/ml and visualized immediately after electrophoresis.

Autres remarques

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Informations sur la sécurité

• Pictogrammes: GHS06,GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H331,H341
• Conseils de prudence: P202 - P261 - P271 - P280 - P304 + P340 + P311 - P308 + P313
• Classification des risques: Acute Tox. 3 Inhalation - Muta. 2
• Code de la classe de stockage: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable