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D3252

Sigma-Aldrich

o-Dianisidine dihydrochloride

≥95%

Synonyme(s) :

3,3′-Dimethoxybenzidine dihydrochloride, Fast Blue B

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About This Item

Formule empirique (notation de Hill):
C14H16N2O2 · 2HCl
Numéro CAS:
20325-40-0
Poids moléculaire :
317.21
Numéro Beilstein :
3917996
Numéro CE :
243-737-5
Numéro MDL:
MFCD00012488
Code UNSPSC :
12171500
ID de substance PubChem :
24893736
Nomenclature NACRES :
NA.47

Niveau de qualité

200

Pureté

≥95%

Forme

powder

Pf

268 °C

Solubilité

H2O: soluble 25 mg/mL

Application(s)

diagnostic assay manufacturing
hematology
histology

Température de stockage

2-8°C

Chaîne SMILES 

Cl.Cl.COc1cc(ccc1N)-c2ccc(N)c(OC)c2

InChI

1S/C14H16N2O2.2ClH/c1-17-13-7-9(3-5-11(13)15)10-4-6-12(16)14(8-10)18-2;;/h3-8H,15-16H2,1-2H3;2*1H

Clé InChI

UXTIAFYTYOEQHV-UHFFFAOYSA-N

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Catégories apparentées

Spécificité

o-Dianisidine (3,3′-dimethoxybenzidine) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is yellow-orange in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 5 M HCl.

Application

o-Dianisidine (3,3′-dimethoxybenzidine) has been used as a component of GO (glucose oxidase) reagent, which is used for measuring the glucose content. It has also been used as a substrate to measure myeloperoxidase activity.

Avertissement

Probably carcinogenic.

Forme physique

crystalline

Pictogrammes

Health hazardCorrosionExclamation mark
GHS08,GHS05,GHS07

Mention d'avertissement

Danger

Mentions de danger

H302,H314,H350

Classification des risques

Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Skin Corr. 1

Code de la classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges

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Certificats d'analyse (COA)

Lot/Batch Number
MKCW7554
MKCW1665
MKCT8252
MKCS4253
SHBN9698

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Luminol-enhanced chemiluminescence induced in peripheral blood-derived human phagocytes: obligatory requirement of myeloperoxidase exocytosis by monocytes.
Albrecht D and Jungi TW
Journal of Leukocyte Biology, 54, 300-300 (1993)
Peter M Meggyesy et al.
Molecules (Basel, Switzerland), 25(21) (2020-10-31)
The therapeutic utility of the copper ionophore disulfiram was investigated in a diet-induced obesity mouse model (C57BL/6J background), both through administration in feed (0.05 to 1% (w/w)) and via oral gavage (150 mg/kg) for up to eight weeks. Mice were
Zhiquan Chen et al.
International journal of molecular sciences, 21(15) (2020-07-28)
Intestinal ischemia reperfusion injury (IRI) is an inherent, unavoidable event of intestinal transplantation, contributing to allograft failure and rejection. The inflammatory state elicited by intestinal IRI is characterized by heightened leukocyte recruitment to the gut, which is amplified by a
Wei Shen et al.
Biosensors & bioelectronics, 44, 171-176 (2013-02-22)
Herein we report a label-free microRNA (miRNA) biosensor in which the formation of a thin insulating film is used to amplify the analytical signal. Briefly, the biosensor is made of an oligonucleotide-coated gold electrode. After hybridizing with a target miRNA
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