Perfect for isolating colonies in cell culture under sterile conditions. The adhesive grease doesn't affect the cells and the plate from which colonies are derived can be left for further cell growth. Easy and straightforward protocol. Used 75uL Trypsin for dissociation and 150uL of growth medium for re-suspension. Was able to obtain and expand clones for analysis.
C1059
Cloning cylinders, glass
Cloning cylinders, glass
1 of 1 reviewers received a sample product or took part in a promotion
volume 150 μL
Synonyme(s) :
cloning cylinder, cloning equipment
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1 EA
96,00 $
96,00 $
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1 EA
96,00 $
About This Item
Code UNSPSC :
41106618
Nomenclature NACRES :
NB.22
96,00 $
En stockDétails
Produits recommandés
Matériaux
light brown (tinted)
Stérilité
sterile; electron beam irradiated
Conditionnement
dish of 15 cylinders
Fabricant/nom de marque
Millipore TR1004
Taille
8 mm × 8 mm
Volume
150 μL
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Description générale
Individual colonies of transfected cells can be isolated and picked from a plate containing many clones. Isolated clones can be dissociated and passaged free from surrounding cells or pulsed with 50-100 uL growth medium which can be analyzed for secreted products. The sterile cloning cylinders are greased on one end to allow the cylinder to seal to the plate. Cells are then dissociated within the cylinder, resuspeneded, and transferred to a new vessel as a pure colony.
These cloning cylinders are sterlized using electron-beam irradiation. The glass cylinders will display a brown tint after being sterlized at the appropriate dosage level.
These cloning cylinders are sterlized using electron-beam irradiation. The glass cylinders will display a brown tint after being sterlized at the appropriate dosage level.
Conditionnement
Packaged in 60 mm dish with silicone grease on one end
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