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A7981

Sigma-Aldrich

Anti-Aromatase antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Anti-ARO, Anti-CYP XIX, Anti-CYP19A1 (Cytochrome P450, Family 19, Subfamily A, Polypeptide 1), Anti-Estrogen Synthase, Anti-P-450 Aromatase

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About This Item

Code UNSPSC :
12352203
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen 50-55 kDa

Espèces réactives

human

Concentration

~1.0 mg/mL

Technique(s)

indirect immunofluorescence: 0.5-1 μg/mL using paraformaldehyde fixed HEK-293T cells transfected with Aromatase
western blot: 0.5-1 μg/mL using human placenta tissue lysate

Numéro d'accès UniProt

P11511

Conditions d'expédition

dry ice

Température de stockage

−20°C

Informations sur le gène

human ... CYP19A1(1588)

Description générale

The enzyme aromatase is a member of P450 cytochrome family. Aromatase catalyzes three oxidation reactions of androstenedione A that lead to aromatization of androgens and subsequent synthesis of estrogen. The enzymatic complex of aromatase is a heterocomplex of cytochrome 450 aromatase and NADPH cytochrome P450 reductase.[1][2] The tissues that produce aromatase are placenta, ovary, brain, bone, adipose tissue, skin and testis.[3] The aromatase enzyme complex is present in the smooth endoplasmic reticulum of cells of these tissues.[4] High levels of aromatase and estrogen are produced by the breast cancer cells. Selective aromatase inhibitors are developed to control the recurrence and survival of hormone-dependent breast cancer tissue[1]
Anti-Aromatase specifically recognizes human aromatase (50-55 kDa).

Application

Anti-Aromatase may be used for immunoblotting at a working concentration of 0.5-1 μg/ml using lysate of human placenta tissue. The antibody has also been used for immunoblotting using whole extracts[5] or nuclear extracts[6] of human primary trophoblasts. For immunofluorescence, a working concentration of 0.5-1 μg/ml is recommended.

Description de la cible

Aromatase is part of the cytochrome p450 family of monooxygenases. It is involved in the production of estrogen and found localized to the endoplasmic reticulum of multiple cell types. [7]

Forme physique

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificats d'analyse (COA)

Lot/Batch Number
020M4821
028K4776

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Saranya Chumsri et al.
The Journal of steroid biochemistry and molecular biology, 125(1-2), 13-22 (2011-02-22)
Estrogens are known to be important in the growth of breast cancers in both pre and postmenopausal women. As the number of breast cancer patients increases with age, the majority of breast cancer patients are postmenopausal women. Although estrogens are
Christos Symeonides et al.
Nature communications, 15(1), 6367-6367 (2024-08-08)
Male sex, early life chemical exposure and the brain aromatase enzyme have been implicated in autism spectrum disorder (ASD). In the Barwon Infant Study birth cohort (n = 1074), higher prenatal maternal bisphenol A (BPA) levels are associated with higher ASD symptoms
Serge Carreau et al.
Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 365(1546), 1571-1579 (2010-04-21)
In most mammalian species aromatase is encoded by a single gene (Cyp19), which contains 18 exons, nine of them being translated. In man, the presence of a biologically active aromatase and oestrogen receptors (ERalpha and ERbeta) has been reported in
Yanyan Hong et al.
Molecular and cellular endocrinology, 340(2), 120-126 (2010-10-05)
Aromatase, estrone sulfatase, and 17β-hydroxysteroid dehydrogenase type 1 are involved in the key steps of 17β-estradiol biosynthesis. Structure-function studies of aromatase, estrone sulfatase and 17β-hydroxysteroid dehydrogenase type 1 are important to evaluate the molecular basis of the interaction between these
E A McDonald et al.
Endocrinology, 150(9), 4358-4365 (2009-06-13)
The hormone adiponectin has been shown to be important in maintaining insulin sensitivity throughout the body, whereas potential effects on the placenta have not been assessed. Pregnancy constitutes a unique physiological environment in which metabolism has a profound effect on
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