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Sigma-Aldrich

Denhardt′s solution

BioReagent, for molecular biology, lyophilized powder

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About This Item

Numéro MDL:
MFCD00717549
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.52

Qualité

for molecular biology

Gamme de produits

BioReagent

Forme

lyophilized powder

Impuretés

DNases, none detected
 RNases, none detected
 phosphatases, none detected
 proteases, none detected

Adéquation

suitable for nucleic acid hybridization

Température de stockage

2-8°C

Description générale

Denhardt′s solution is a mixture of high-molecular weight polymers that act as blocking agents. It is used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This powder can be used to make a 50x concentrate that can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.

Application

Suitable to block non-specific binding sites in western, northern and Southern blot membranes

Composants

Each vial of Denhardt′s powder contains 50 mG Bovine Serum Albumin (BSA), 50 mG Ficoll, and 50 mG Polyvinylpyrrolidone (PVP).

Notes préparatoires

To make a 50x concentrate, dissolve entire contents of vial in 5 mL molecular biology grade water. Store at −20°C for up to two years.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

P7059

Solution tampon phosphate, 10× PBS for Western blots and IP

B6429

Casein Blocking Buffer 10x, for Northern and Southern blotting, solution

H7140

Hybridization Solution, for Northern and Southern blotting, liquid (2x concentrate)

M5755

MOPS-EDTA-Sodium Acetate (MESA), powder for RNA electrophoresis buffers

H7033

Tampon d′hybridation PerfectHyb™ Plus, for Northern and Southern blotting, solution

D9905

Denhardt′s Solution, for Northern and Southern blotting, lyophilized powder

D2532

Denhardt′s Solution 50x, for Northern and Southern blotting, solution

P1415

Prehybridization Solution, for Northern and Southern blotting, liquid (2x concentrate)

C7594

Casein Blocking Buffer, for Northern and Southern blotting, powder blend

93017

SSC Buffer 20x, for Northern and Southern blotting, solution

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Flavien Pillet et al.
Nucleic acids research, 39(17), 7477-7486 (2011-06-10)
The segregation of plasmid F of Escherichia coli is highly reliable. The Sop partition locus, responsible for this stable maintenance, is composed of two genes, sopA and sopB and a centromere, sopC, consisting of 12 direct repeats of 43 bp. Each
J. Sambrook et al.
Molecular Cloning: A Laboratory Manual (1989)
Ren Li et al.
Nature communications, 11(1), 5844-5844 (2020-11-19)
Fruit firmness is a target trait in tomato breeding because it facilitates transportation and storage. However, it is also a complex trait and uncovering the molecular genetic mechanisms controlling fruit firmness has proven challenging. Here, we report the map-based cloning

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