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M5755

Sigma-Aldrich

MOPS-EDTA-Sodium Acetate (MESA)

powder for RNA electrophoresis buffers

Synonyme(s) :

MESA Buffer

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About This Item

Code UNSPSC :
12161700
Nomenclature NACRES :
NA.25

Qualité

for molecular biology

Gamme de produits

BioReagent

Forme

powder

Technique(s)

electrophoresis: suitable

pH

6.8-7.2(reconstituted per directions)

Solubilité

H2O: soluble, faintly yellow

Activité étrangère

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

Température de stockage

room temp

Catégories apparentées

Description générale

MOPS – EDTA – Sodium Acetate (MESA) is the most commonly used running buffer for RNA electrophoresis prior to Northern blotting procedures. Applied voltage of 5 V/cm is recommended for maximum resolution.

Application

Suitable for use when making formaldehyde-agarose gels and associated running buffer for RNA electrophoresis.

Composants

1x MESA buffer contains:
  • 40 mM MOPS
  • 10 mM sodium acetate
  • 1 mM EDTA (pH 8.3)

Reconstitution

Dissolve entire contents of bottle in a final volume of 1L molecular biology grade water. This produces a 10x MESA stock solution that can be further diluted as needed.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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MOPS sodium salt anhydrous, free-flowing, Redi-Dri™, ≥99.5%

Sigma-Aldrich

RDD018

MOPS sodium salt

MOPS, Free Acid, Molecular Biology Grade CAS 1132-61-2 is a zwitterionic buffer useful in the pH range of 6.5-7.9. Has a pKa of 7.20 at 25°C.

Millipore

475922

MOPS, Free Acid, Molecular Biology Grade

MOPS anhydrous, free-flowing, Redi-Dri™, ≥99.5%

Sigma-Aldrich

RDD003

MOPS

Kathryn M Jones et al.
Journal of bacteriology, 184(9), 2491-2499 (2002-04-12)
Two operons have been cloned from Anabaena sp. strain PCC 7120 DNA, each of which encodes the three core subunits of distinct mitochondrial-type cytochrome c oxidases. The two operons are only 72 to 85% similar to one another at the
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 7-7 (1989)
Xueyu Xiang et al.
Developmental biology, 275(2), 343-355 (2004-10-27)
Recently, we cloned and sequenced the cDNA of allurin, a sperm chemoattractant isolated from the jelly of Xenopus laevis eggs [Proc. Natl. Acad. Sci. U.S.A. 78 (2001) 11205]. In this report, we demonstrate that allurin mRNA is expressed almost exclusively
Molecular techniques for detection of gene expression in neuroimmunology.
Cowan, E.P., and Dhib-Jalbut, S.S.
Methods in Neuroscience, 24, 41-41 (1995)
Electrophoresis in agarose and acrylamide gels.
R C Ogden et al.
Methods in enzymology, 152, 61-87 (1987-01-01)
Afficher tous les articles scientifiques apparentés

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