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Millipore

2,3,5-Triphenyl-tetrazolium chloride solution

suitable for microbiology, Filter sterilized solution that is recommended for the detection of microbial growth based on reduction of TTC

Synonyme(s) :

TTC solution, TTC supplement, TTC solution, TTC supplement

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About This Item

Formule empirique (notation de Hill):
C19H15ClN4
Numéro CAS:
Poids moléculaire :
334.80
Numéro Beilstein :
3923356
Numéro MDL:
Code UNSPSC :
41171614
ID de substance PubChem :
Nomenclature NACRES :
NA.85

Niveau de qualité

Stérilité

sterile (Filtered and Aseptic Handled)

Forme

liquid

Durée de conservation

limited shelf life, expiry date on the label

Poids mol.

334.8 g/mol

Application(s)

agriculture
environmental
food and beverages

microbiology

Température de stockage

2-8°C

Adéquation

bacteria

Chaîne SMILES 

[Cl-].c1ccc(cc1)-c2nn(-c3ccccc3)[n+](n2)-c4ccccc4

InChI

1S/C19H15N4.ClH/c1-4-10-16(11-5-1)19-20-22(17-12-6-2-7-13-17)23(21-19)18-14-8-3-9-15-18;/h1-15H;1H/q+1;/p-1

Clé InChI

PKDBCJSWQUOKDO-UHFFFAOYSA-M

Description générale

2,3,5-Triphenyl-tetrazolium chloride (TTC) is a dye largely used for the easy enumeration of microbial colonies in solid culture media. This dye is colorless in the oxidized form and changes to red when reduced by microorganisms, due to the formation of formazan. Live microorganisms, for example, E. coli and other coliforms reduce TTC by enzymatic action, forming red color formazan that is kept inside granules in the cells. TTC is highly recommended in testing pasteurized milk because there is a high percentage of microorganisms unable to reduce TTC in pasteurized milk, which cannot be detected by laboratory procedures. Higher concentrations of TTC may have an inhibitory effect on the growth of Gram-positive organisms.

Application

2,3,5-Triphenyl-tetrazolium chloride (TTC) solution is a redox indicator that can help indicate cellular respiration and identify viable and non-viable cells as well as identify microorganisms capable of reduction of TTC on solid culture media

Composants

(per vial)
2,3,5-Triphenyl tetrazolium chloride: 0.1 g, distilled water: 10 ml

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves


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