10269611001
Roche
Neuraminidase (Sialidase)
from Arthrobacter ureafaciens
Synonyme(s) :
PCR, taq
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About This Item
Produits recommandés
Source biologique
bacterial (Arthrobacter ureafaciens)
Niveau de qualité
Forme
solution
Activité spécifique
~25 units/mg protein
Conditionnement
pkg of 1 U (100 μl)
Fabricant/nom de marque
Roche
pH optimal
5.0-5.5
Température de stockage
2-8°C
Description générale
Neuraminidase hydrolyzes terminal N- or O-acylneuraminic acids which are α2,3-, α2,6-, or α2,8-linked (rate: α2,6 > α2,3 > α2,8) to oligosaccharides, polysaccharides, mucopolysaccharides, glycoproteins, and glycolipids. Noteworthy, for the hydrolysis of glycolipids, the presence of a detergent is necessary. Because of the broad substrate specificity, the enzyme is very well suited for the complete removal of sialic acids from glycoconjugates of a wide variety of biological materials.
Spécificité
Cleaves terminal sialic-acid residues that are α2,3-, α2,6-, or α2,8-linked to Gal, GlcNAc, GalNAc, AcNeu, GlcNeu, oligosaccharides, glycolipids, or glycoproteins. Relative rate of cleavage is α2,6 >α2,3 >α2,8, determined on bonds in tri- and tetrasaccharides.
Application
- cell surface lectin array analysis.
- hemagglutination assays.
- cell adhesion assay.
For the hydrolysis of glycolipids, the presence of a detergent is necessary. Because of the broad substrate specificity, the enzyme is very well suited for the complete removal of sialic acids from glycoconjugates of a wide variety of biological materials.
Neuraminidase has been used for the:
- detection of the cell surface glycosylations in human anaplastic large cell lymphoma cells
- release of sialic acid from cells
- antibody-overlay lectin microarray
Propriétés physiques
This product is a mixture of isoenzymes (L, M1, M2 and S) with the following molecular weight values: ~ 52 kDa, 66 kDa and 88 kDa.
Forme physique
Solution in 10 mM sodium phosphate, 0.1% Micr-O-Protect (w/v), 0.25 mg/ml bovine serum albumin, pH 7
Notes préparatoires
Working concentration: Enzyme/substrate ratio should be in the range of 0.04 U/25-80 μg.
Autres remarques
For life science research only. Not for use in diagnostic procedures.
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Skin Sens. 1
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
does not flash
Point d'éclair (°C)
does not flash
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Protocoles
Neuraminidase can be used to cleave sialic acids from proteins. In this protocol, the enzyme from Vibrio cholerae is used on fixed cells.
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