2750

BrdU Cell Proliferation Kit

The BrdU Cell Proliferation Assay Kit is used to detect the BrdU in a sample.

• Synonyme(s) : Bromodeoxyuridine Assay, non isotopic assay
• Code UNSPSC : 12161503
• Nomenclature NACRES : NA.76

Propriétés

• Niveau de qualité: 100
• Espèces réactives: horse, guinea pig, rat, human, canine, mouse, pig, rabbit, hamster, nonhuman primates
• Conditionnement: pkg of 200 assay(s)
• Fabricant/nom de marque: Chemicon^®
• Technique(s): ELISA: suitable
• Méthode de détection: colorimetric

Description

Description générale

Test Principle With CHEMICON^®′s BrdU Cell Proliferation Assay Kit, mouse monoclonal antibody is used to detect the BrdU in a sample. After addition of goat anti-mouse IgG-peroxidase conjugated secondary antibody, substrate and stop solution, the amount of BrdU is determined. The higher the OD, the higher the BrdU concentration in the sample.

Application

The BrdU Cell Proliferation Assay Kit is used to detect the BrdU in a sample.

The CHEMICON^® BrdU Cell Proliferation Assay Kit is a non-isotopic assay for the in vitro quantitative detection of newly synthesized DNA of actively proliferating cells. For Research Use Only. Not for use in diagnostic procedures.

Conditionnement

200 assays

Composants

• BrdU Reagent: (Part No. 2750a) One 15 μL vial of a 500X solution of BrdU.
• Fixing Solution: (Part No. 2750b) One 40 mL bottle.
• Prediluted BrdU Detection Antibody: (Part No. 2750c) One 20 mL vial of an anti-BrdU solution.
• Stop Solution: (Part No. 2750d): One 25 mL bottle of 2.5N sulfuric acid solution.
• Goat anti-Mouse IgG, Peroxidase labeled: (Part No. 2750e) One 15 μL vial of a 2000X solution.
• Conjugate Diluent: (Part No. 2750f) One 25 mL bottle.
• Substrate: (Part No. 2750g) One 25 mL bottle, ready to use TMB.
• 50X Plate Wash Concentrate: (Part No. 2750h) One 90 mL bottle of buffered Tris with surfactant.

Stockage et stabilité

Maintain the kit at 2° to 8°C under sterile conditions for up to 6 months after date of receipt.

Before first use: remove the Fixative Solution and place at room temperature for at least 4 hours prior to use. Precipitates that may occur during cold storage should go back into solution.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids