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Supelco

SeQuant® ZIC®-cHILIC 100Å (3 µm) HPLC Columns

L x I.D. 100 mm x 2.1 mm HPLC Column, PEEK-lined stainless steel

Synonyme(s) :

Zwitterionic Hydrophilic Interaction Liquid Chromatography HPLC Column

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About This Item

Code UNSPSC :
41115709
Nomenclature NACRES :
SB.52

product name

SeQuant® ZIC®-cHILIC 3µm,100Å 100 x 2.1 mm, PEEK-lined stainless steel column, suitable for HPLC

Matériaux

PEEK-lined stainless steel column

Niveau de qualité

100

Gamme de produits

SeQuant®

Caractéristiques

endcapped: no

Paramètres

550 bar pressure
70 °C max. temp.

Technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
mass spectrometry (MS): suitable (High)

Colonne, L × D.I.

10 cm × 2.1 mm

Matrice

fully porous particle

Groupe de la matrice active

ZIC-cHILIC bonding phase

Taille des particules

3 μm

Dimension de pores

100 Å pore size

pH de fonctionnement

1-7.5

Technique de séparation

hydrophilic interaction (HILIC)

Température de stockage

no temp limit

Catégories apparentées

Description générale

SeQuant® ZIC®-cHILIC is designed for excellent HPLC and LC-MS of polar hydrophilic compounds. This zwitterionic stationary phase with phosphorylcholine functional group provides you with complementary selectivity for easier method development for substances that have been difficult to separate by previous types of HPLC columns operated in reversed-phase or HILIC mode.The column dimension of 100 mm length x 2.1 mm ID is the best choice for the separation of polar compounds using a UHPLC or optimized HPLC Instrument providing low dwell volumes.SeQuant® ZIC®-cHILIC HPLC columns provide complementary selectivity to optimize separations of polar hydrophilic compounds. The columns carry a novel densely bonded, 1:1 charge-balanced phosphorylcholine functional group. As with all true zwitterionic stationary phases, straightforward and reproducible separations are achieved by hydrophilic partitioning combined with weak ionic interactions.The more accessible positive charges of SeQuant® ZIC®-cHILIC columns enable selectivity tuning in chromatography through altered interaction with anionic and cationic molecular moieties. This can be favorable in several situations, for example in the separation of labile metal complexes or highly polar and multiply charged aminoglycosides. Their extraordinary stability and minimized bleed also make SeQuant® ZIC®-cHILIC columns the preferred choice for many LC-MS applications.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development Guide

Remarque sur l'analyse

Pressure: ≤ 40 bar
Theoretical Plates (N/m) (Cytosine): ≥ 90000
Symmetry (Cytosine): 0.95 - 1.25

Informations légales

SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany

Produit(s) apparenté(s)

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Description
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Certificats d'analyse (COA)

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Articles

Selectivity Examination of Stationary Phases for HILIC

This article evaluates different phase chemistries for stationary phases in Hydrophilic Interaction Liquid Chromatography (HILIC) and uses multivariate analysis for classification based on chemical modification.

Protocoles

LC/MS Analysis of Nucleotides SeQuant® ZIC®-cHILIC – Effect of pH

ZIC®-cHILIC is a densely bonded zwitterionic stationary phase with phosphorylcholine functional groups covalently attached to silica.

LC/MS Analysis of Nucleotides on SeQuant ZIC-cHILIC

HILIC separation is an alternative that permits sensitive MS detection and without the use of ion-pair reagents.

Contenu apparenté

Small Molecule HPLC

Small molecules are ions and compounds of molecular weight typically less than 900 daltons. These compounds can be effectively separated and analyzed by HPLC, UHPLC and LC-MS using mainly silica particles or monolithic stationary phases with a broad range of column chemistries (modifications).

HPLC de petites molécules

Les petites molécules sont des ions ou des composés d'une masse moléculaire généralement inférieure à 900 daltons. Ces molécules peuvent être séparées efficacement et analysées par HPLC, UHPLC et LC-MS en utilisant essentiellement des particules de silice ou des phases stationnaires monolithiques présentant un large éventail de chimies de colonnes (modifications) différentes.

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