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581510-U

Supelco

Ascentis® Si (5 µm) HPLC Columns

L × I.D. 25 cm × 2.1 mm, HPLC Column

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About This Item

Código UNSPSC:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Ascentis® Si HPLC Column, 5 μm particle size, L × I.D. 25 cm × 2.1 mm

Materiais

stainless steel column

Agency

suitable for USP L3

linha de produto

Ascentis®

Características

endcapped: no

fabricante/nome comercial

Ascentis®

embalagem

1 ea of

Parâmetros

≤70 °C temp. range
400 bar pressure (5801 psi)

técnica(s)

HPLC: suitable
LC/MS: suitable

C × D.I.

25 cm × 2.1 mm

área da superfície

450 m2/g

Impurezas

<5 ppm metals

matriz

fully porous particle
silica gel high purity, spherical

Grupo ativo da matriz

silica phase

tamanho de partícula

5 μm

tamanho de poro

100 Å

operating pH range

2-6

aplicação(ões)

food and beverages

técnica de separação

hydrophilic interaction (HILIC)
normal phase

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Descrição geral

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

The Ascentis Si is a high loading capacity silica with excellent peak shape. The Ascentis Si performs in both normal-phase and HILIC/ANP (aqueous normal phase) mode.

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Informações legais

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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Certificados de análise (COA)

Lot/Batch Number
279115-01
278999-01
278974-01
278877-01
278106-01

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Deborah Benhaim et al.
Journal of chromatography. A, 1217(1), 65-74 (2009-11-27)
This study investigates lipophilicity determination by chromatographic measurements using the polar embedded Ascentis RP-Amide stationary phase. As a new generation of amide-functionalized silica stationary phase, the Ascentis RP-Amide column is evaluated as a possible substitution to the n-octanol/water partitioning system
Shigehisa Uchiyama et al.
Analytical chemistry, 80(9), 3285-3290 (2008-03-18)
A new method for the simultaneous determination of ozone and carbonyls in air using a two-bed cartridge system has been developed. Each bed consists of reagent-impregnated silica particles. The first contains trans-1,2-bis-(4-pyridyl) ethylene (BPE) while the second contains 2,4-dinitrophenylhydrazine (DNPH).
Paweł Kubica et al.
Journal of chromatography. A, 1289, 13-18 (2013-04-04)
Two analytical procedures are proposed where HILIC and RPLC techniques are coupled with tandem mass spectrometry detection for rapid determination of trace amounts of nicotine in zero-level liquids for electronic cigarettes. Samples are prepared on the basis of the approach
Szabolcs Fekete et al.
Journal of pharmaceutical and biomedical analysis, 50(5), 703-709 (2009-06-30)
The performance of a narrow bore silica based monolith column (5 cm x 2 mm) was compared to 5 cm long narrow bore (internal diameter < or = 2.1 mm) columns, packed with shell particles (2.7 microm) and totally porous
Szabolcs Fekete et al.
Journal of pharmaceutical and biomedical analysis, 49(1), 64-71 (2008-11-29)
The performance of 5 cm long columns packed with shell particles was compared to totally porous sub-2 microm particles in gradient and isocratic elution separations of hormones (dienogest, finasteride, gestodene, levonorgestrel, estradiol, ethinylestradiol, noretistherone acetate, bicalutamide and tibolone). Peak capacities
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