Pular para o conteúdo
Merck
Todas as fotos(1)

Documentos Principais

567536-U

Supelco

Discovery® HS F5 (5 µm) HPLC Columns

L × I.D. 25 cm × 4 mm, HPLC Column

Sinônimo(s):

Discovery Pentafluorophenyl PFP HPLC Column

Faça loginpara ver os preços organizacionais e de contrato


About This Item

Código UNSPSC:
41115700
eCl@ss:
32110501
NACRES:
SB.52

Nome do produto

Discovery® HS F5 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4 mm

Materiais

stainless steel column

Nível de qualidade

Agency

suitable for USP L43

linha de produto

Discovery®

Características

endcapped

fabricante/nome comercial

Discovery®

embalagem

1 ea of

Extensão da rotulagem

12% Carbon loading

Parâmetros

≤70 °C temp. range
400 bar pressure (5801 psi)

técnica(s)

HPLC: suitable
LC/MS: suitable

C × D.I.

25 cm × 4 mm

área da superfície

300 m2/g

cobertura de superfície

4 μmol/m2

Impurezas

<10 ppm metals

Matriz

silica gel, high purity, spherical particle platform
fully porous particle

Grupo ativo da matriz

PFP (pentafluorophenyl) phase

tamanho de partícula

5 μm

tamanho de poro

120 Å

Faixa de pH operacional

2-8

aplicação(ões)

food and beverages

técnica de separação

reversed phase

Procurando produtos similares? Visita Guia de comparação de produtos

Descrição geral

Guidelines for transferring a C18 method to Discovery® HS F5:
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log Po/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.
The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.

Características e benefícios

  • Unique selectivity
  • Similar retention to C18 (sometimes requires stronger mobile phase)
  • Excellent peak shape
  • Stable, low-bleed LC-MS separations
  • Scalable separations from 3 to 10μm particle sizes

Produtos recomendados

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Informações legais

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Escolha uma das versões mais recentes:

Certificados de análise (COA)

Lot/Batch Number

Não está vendo a versão correta?

Se precisar de uma versão específica, você pode procurar um certificado específico pelo número do lote ou da remessa.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Jennie Wang et al.
Journal of pharmaceutical and biomedical analysis, 124, 267-273 (2016-03-13)
An HPLC method for the assay of a DNA topoisomerase inhibitor, LMP776 (NSC 725776), has been developed and validated. The stress testing of LMP776 was carried out in accordance with International Conference on Harmonization (ICH) guidelines Q1A (R2) under acidic
Umberto M Battisti et al.
Journal of chromatography. A, 1443, 152-161 (2016-03-30)
A "heart-cut" two-dimensional achiral-chiral liquid chromatography triple-quadrupole mass spectrometry method (LC-LC-MS/MS) was developed and coupled to in vivo cerebral microdialysis to evaluate the brain response to the chiral compound (±)-7-chloro-5-(3-furanyl)-3-methyl-3,4-dihydro-2H-1,2,4-benzothiadiazine-1,1-dioxide ((±)-1), a potent positive allosteric modulator (PAM) of AMPA receptor.
Lucie Havlíková et al.
Talanta, 76(3), 597-601 (2008-07-01)
A pentafluorophenylpropyl (PFPP) stationary phase was for the first time tested for the simultaneous determination of triamcinolone acetonide, its degradation product triamcinolone and two preservatives, methylparaben, and propylparaben. A new simple isocratic reversed phase HPLC method with UV detection, using
Jana Maláková et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 853(1-2), 265-274 (2007-04-10)
Galantamine, an alkaloid isolated from the bulbs and flowers of Caucasian snowdrop (Galanthus woronowii, Amaryllidaceae) and related species, is employed in human medicine for the treatment of various neuromuscular and neurodegenerative diseases. After the administration, the products of oxidative biotransformation
Ilia Brondz et al.
Journal of pharmaceutical and biomedical analysis, 43(3), 937-944 (2006-11-03)
The drug primaquine diphosphate is used for causative treatment of malaria. Using HPLC-MS and GC-MS, this research group was previously able to show that the main contaminant of primaquine is the positional isomer quinocide [I. Brondz, D. Mantzilas, U. Klein

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica