Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize product 53299-U Ascentis® Express Peptide ES C18 HPLC column efficiency. The suggested starting point for flow rate for Ascentis® Express columns: 1.6 mL/min for 4.6 mm ID; 0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.
53299-U
Ascentis® Express Peptide 160 Å ES-C18 (2.7 μm) HPLC Columns
L × I.D. 3 cm × 2.1 mm, HPLC Column
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About This Item
Código UNSPSC:
41115700
eCl@ss:
32110501
NACRES:
SB.52
Preço e disponibilidade não estão disponíveis no momento.
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Nome do produto
Ascentis® Express Peptide ES-C18, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 3 cm × 2.1 mm
Materiais
stainless steel column
Nível de qualidade
Agency
suitable for USP L1
linha de produto
Ascentis®
Características
endcapped: no
fabricante/nome comercial
Ascentis®
embalagem
1 ea of
Parâmetros
≤100 °C temp. range
600 bar max. pressure (9000 psi)
técnica(s)
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
C × D.I.
3 cm × 2.1 mm
área da superfície
90 m2/g
Impurezas
<5 ppm metals
Matriz
Fused-Core particle platform
superficially porous particle
Grupo ativo da matriz
C18 (octadecyl) phase
tamanho de partícula
2.7 μm
tamanho de poro
160 Å
Faixa de pH operacional
1-9
técnica de separação
reversed phase
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Categorias relacionadas
Descrição geral
Ascentis Express Peptide ES-C18 columns are specifically engineered to separate higher molecular weight compounds such as peptides and small proteins. These columns contain advanced Fused-Core particles that have larger pores (160 Å versus 90 Å in standard Ascentis Express), bonded with sterically-protected C18 ligands to provide extra stability (ES) at very low pH (< 1) and high temperatures (up to 100ºC). This greatly expands the application range for Ascentis Express columns.
Informações legais
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
necessário, mas não fornecido
Nº do produto
Descrição
Preços
produto relacionado
pré-cartucho
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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Meiyun Shi et al.
Journal of separation science, 38(8), 1351-1357 (2015-01-30)
The pentapeptide thymopentin (Arg-Lys-Asp-Val-Tyr, RKDVY) corresponds to amino acids 32-36 of the 49 amino acid immunomodulatory polypeptide, thymopoietin, whose biological activity is partially reproduced. Thymopentin is widely used in the clinic and represents a promising target for drug design but
E Lesellier
Journal of chromatography. A, 1266, 34-42 (2012-11-03)
The recent introduction of new stationary phases for liquid chromatography based on superficially porous particles, called core-shell or fused-core, dramatically improved the separation performances through very high efficiency, due mainly to reduced eddy diffusion. However, few studies have evaluated the
C Gröer et al.
Journal of pharmaceutical and biomedical analysis, 100, 393-401 (2014-09-15)
Cytochrome P450 (CYP) enzymes and UDP-glucuronosyltransferases (UGT) are major determinants in the pharmacokinetics of most drugs on the market. To investigate their impact on intestinal and hepatic drug metabolism, we developed and validated quantification methods for nine CYP (CYP1A2, CYP2B6
Artigos
Choose Ascentis® Express Peptide ES-C18 U/HPLC Columns based on Fused-Core® technology for fast and efficient separation of high-molecular weight compounds, such as peptides and small proteins. With a 2.7 µm particle size and rigorous testing, these columns offer reliable results and high efficiency.
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What flow rate should I use with product 53299-U Ascentis® Express Peptide ES C18 HPLC columns?
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Can I use product 53299-U Ascentis® Express Peptide ES C18 HPLC Column on any type of HPLC system?
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Product 53299-U Ascentis® Express Peptide ES C18 HPLC Column HPLC columns is capable of use on standard HPLC systems as well as UHPLC systems. This column is packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.
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Do I need special fittings and tubing to connect product 53299-U Ascentis® Express Peptide ES C18 HPLC ColumnExpress columns?
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While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.
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What is the carbon load for product 53299-U Ascentis® Express Peptide ES C18 HPLC Column?
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Carbon load which is commonly referred to as %Carbon is misleading and not meaningful for Ascentis Express. Since Fused-Core™ particle is unique in the sense that it has a solid core and porous shell, the %Carbon number can not be compared to traditional porous particles. The carbon coverage for product 53299-U Ascentis® Express Peptide ES C18 HPLC column is 2 micromoles/meter-squared.
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How can I measure my instrument bandwidth (IBW) and determine what columns can be used with minimal efficiency loss created by too much internal instrument volume?
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The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.
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Is there anything special I need to do to my HPLC system to use product 53299-U Ascentis® Express Peptide ES C18 HPLC Column?
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Nothing special is required to use product 53299-U Ascentis® Express Peptide ES C18 HPLC Column. To obtain the full benefits of Ascentis® Express Column, one should minimize dispersion or instrument bandwidth in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level. For more information, request Guidelines for Optimizing Performance with Ascentis® Express HPLC Columns (T407102).
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Can product 53299-U Ascentis® Express Peptide ES C18 HPLC column be used for LC-MS?
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Yes, Express Fused-Core™ particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of product 53299-U Ascentis® Express Peptide ES C18 HPLC Column for high throughput UHPLC and LC-MS is that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.
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Can I use product 53299-U Ascentis® Express Peptide ES C18 HPLC Column on a UHPLC system?
1 answer-
Yes. product 53299-U Ascentis® Express Peptide ES C18 HPLC columns are packed in a way making them suitable for these ultra high pressure instruments. In fact, product 53299-U Ascentis® Express Peptide ES C18 HPLC Column outperforms sub-2 μm micron columns on many applications since product 53299-U Ascentis® Express Peptide ES C18 HPLC Column provides the benefits of sub-2 μm particles but at much lower back pressure. These benefits include the capability of providing fast HPLC and higher resolution chromatography. The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell. A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
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What is the pressure rating for product 53299-U Ascentis® Express Peptide ES C18 HPLC Column?
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This product 53299-U Ascentis® Express Peptide ES C18 HPLC Column is stable to operating pressures up to 600 bar (9000 psi)
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Are guard columns available for product 53299-U Ascentis® Express Peptide ES C18 HPLC Column?
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Yes, we have guard columns available for product 53299-U Ascentis® Express Peptide ES C18 HPLC Column. Product 53500-U is the Ascentis® Express Guard Holder and Product 53536-U Ascentis® Express Peptide ES C18 Guard Cartridge is a package of three guard cartridges, 2.7 μm particle size, L 5mm × I.D. 2.1 mm .
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