ZRB1899

Anti-Perilipin-1 Antibody, clone 1G23 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Sinônimo(s): Lipid droplet-associated protein
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 200
• recombinante: expressed in HEK 293 cells
• conjugado: unconjugated
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: 1G23, recombinant monoclonal
• descrição: 1G23 Clone
• linha de produto: ZooMAb^® learn more
• forma: lyophilized
• peso molecular: calculated mol wt 55.99 kDa
• purificado por: using Protein A
• reatividade de espécies: human
• embalagem: antibody small pack of 25 μL
• características do produto alternativo mais ecológico: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• validação aprimorada: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• técnica(s): affinity binding assay: suitable; flow cytometry: suitable; immunohistochemistry: suitable
• Isotipo: IgG
• sequência de epítopo: C-terminal
• nº de adesão de ID de proteína: NP_001138783
• nº de adesão UniProt: O60240
• categoria alternativa mais ecológica: Aligned,
• Condições de expedição: ambient
• temperatura de armazenamento: 2-8°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... PLIN1(5346)

Descrição

Descrição geral

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Especificidade

Clone 1G23 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Perilipin-1. It targets an epitope within 21 amino acids from the C-terminal region.

Imunogênio

KLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal region of human Perilipin-1.

Aplicação

Quality Control Testing

Evaluated by Immunohistochemistry (Paraffin) in Human breast tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution of this antibody detected Perilipin-1 in Human breast tissue sections.

Tested Applications

Affinity Binding Assay: A representative lot of this antibody bound Perilipin-1 peptide with a KD of 1.8 x 10-6 in an affinity binding assay.

Flow Cytometry Analysis: 1 µg from a representative lot detected Perilipin-1 in U2OS cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descrição-alvo

Perilipin-1 (UniProt: O60240; also known as Lipid droplet-associated protein) is encoded by the PLIN1 (also known as PERI, PLIN) gene (Gene ID: 5346) in human. Perilipins are lipid droplet (LD)- binding proteins that are required for optimal lipid storage and fatty acid release. In mammals, the perilipin family of comprises five members: perilipin, adipophilin, TIP47, S3-12 and MLDP/OXPAT (also named PLIN1-5. Perilpin-1 is a modulator of adipocyte lipid metabolism that is detected in adipocytes and is also shown to be expressed in hepatocytes of human steatosis liver. It coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase. It is shown to play a role in unilocular lipid droplet formation by activating cell death inducing DFFA like effector c (CIDEC). Their interaction promotes lipid droplet enlargement and directional net neutral lipid transfer. In adipocytes, perilipin-1 can undergo phosphorylation by protein kinase A that changes its conformation and permits lipolysis by hormone-sensitive lipase. Perilipin knockout mice are lean and display aberrant adipocyte lipolysis, enhanced leptin production, and resistance to diet-induced obesity. Mutations in PLIN1 gene are linked to familial lipodystrophy that is characterized by loss of subcutaneous adipose tissue primarily affecting the lower limbs, insulin-resistant diabetes mellitus, hypertriglyceridemia, and hypertension. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Straub, BK et al. (2008). Hepatology. 47(6); 1936-1946; Tansey JT., et al. (2004). IUBMB Life. 56(7); 379-385).

forma física

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstituição

0.3 mg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Armazenamento e estabilidade

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informações legais

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable