As provided, this product contains 100 units per vial. The mass weight per vial is not determined.
U3383
Urease Buffer Reagent
Sinônimo(s):
Urea colorimetric determination assay reagent
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1 VIAL
R$ 575,00
R$ 575,00
Previsão de entrega em01 de junho de 2025
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1 VIAL
R$ 575,00
About This Item
Código UNSPSC:
12352204
NACRES:
NA.54
R$ 575,00
Previsão de entrega em01 de junho de 2025
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descrição
When reconstituted, product is a solution of urease from Jack bean in 27 mM EDTA with 0.1% sodium azide as a preservative.
Nível de qualidade
Formulário
powder
peso molecular
545 kDa
temperatura de armazenamento
2-8°C
Descrição geral
Urease is a 545 kDa protein critical for the hydrolysis of urea to carbon dioxide and ammonia. [1]
Urease is a dinickel enzyme that hydrolyses urea into ammonia and carbon dioxide.[2][3] It is secreted by several bacterial species, invertebrates, fungi, and plants.[3] Detection of urease activity is a potential diagnostic tool for Helicobacter pylori infections and several oral bacterial infections.[2] two Ni2+ ions bridged by a hydroxyl group and a carbamylated lysine.[3]
Aplicação
Urease Buffer Reagent has been used:
- to measure plasma urea N (PUN) by the hypochlorite-phenol nitroprusside method[4]
- in cell viability assay to measure the synthesis of urea in human fetal stem/progenitor cells[5]
- to study the metabolic capacity of rat hepatocytes[6]
Used in conjunction with Phenol Nitroprusside solution (Product Code P 6994) and Alkaline Hydpochlorite solution (Product Code A 1727) for the colorimetric determination of urea.
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2
Código de classe de armazenamento
13 - Non Combustible Solids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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Kang Cheng et al.
Hepatology (Baltimore, Md.), 50(4), 1194-1203 (2009-07-29)
Tracking stem/progenitor cells through noninvasive imaging is a helpful means of assessing the targeting of transplanted cells to specific organs. We performed in vitro and in vivo studies wherein adult human hepatocytes and human fetal liver stem/progenitor cells were labeled
Gunnar Dahlén et al.
BMC oral health, 18(1), 89-89 (2018-05-20)
Urease is an enzyme produced by plaque bacteria hydrolysing urea from saliva and gingival exudate into ammonia in order to regulate the pH in the dental biofilm. The aim of this study was to assess the urease activity among oral
Yuta Enami et al.
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Phytochemistry, 69(1), 18-28 (2007-08-21)
The broad distribution of ureases in leguminous seeds, as well as the accumulation pattern of the protein during seed maturation, are suggestive of an important physiological role for this enzyme. Since the isolation and characterization of jack bean urease by
L A Winkelman et al.
Journal of dairy science, 96(12), 7565-7577 (2013-10-15)
This study investigated the effect of 2 different types of long-acting insulin on milk production, milk composition, and metabolism in lactating dairy cows. Multiparous cows (n=30) averaging 88 d in milk were assigned to one of 3 treatments in a
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What's the concentration of Jack bean Urease? I can only see the concentration of buffer.
1 answer-
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How many urease units does 1 vial contain?
1 answer-
As provided, this product contains 100 units per vial.
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What is the volume of water used for reconstitution of 1 vial?
1 answer-
As mentioned on the sample Certificate of Analysis, 30 mL of purified water in one vial of this material yields a clear solution.
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