Testing was conducted on a solution at 5 mg/mL in 0.1 M Acetate pH 4.0, as indicated in the Certificate of Analysis. Solution stability and storage conditions have not been tested.
49291
Glucosidase from Aspergillus niger
powder, ≥750 U/g
Sinônimo(s):
Cellobiase
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Produtos recomendados
Formulário
powder
atividade específica
≥750 U/g
temperatura de armazenamento
2-8°C
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Categorias relacionadas
Ações bioquímicas/fisiológicas
Glucosidase catalyzes the hydrolysis of α-1,4 linkages with a substrate preference for maltose, maltotriose and maltotetraose. Reactivity with large polysaccharides like dextrin and starch have also been described.
Definição da unidade
1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol p-nitrophenyl-β-D-glucopyranoside per minute at pH 4.0 and 37 °C; may contain α- and β-glucosidase
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Resp. Sens. 1
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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T Watanabe et al.
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Beta-glucosidase was purified from a crude cellulase preparation from Aspergillus niger by affinity chromatography on a methacrylamide-N-methylene-bis-methacrylamide copolymer bearing cellobiamine. The purified enzyme was a dimer with an isoelectric point of 4.0. The molecular mass of the enzyme was estimated
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What buffer should I reconstitute this enzyme for preparing a stock solution? Or does cold deionized water work? Either way, once resuspended, at what temperature should it be stored? Thank you!
1 answer-
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