T3194

Tetanus toxin

from Clostridium tetani, cleaves synaptobrevin (VAMP-2)

• Número CAS: 676570-37-9
• Número MDL: MFCD01095768
• Código UNSPSC: 12352200
• NACRES: NA.77

Propriedades

• Nome do produto: Tetanus toxin from Clostridium tetani,
• peso molecular: 150 kDa (heavy chain (100 kDa) and a light chain (50 kDa) connected through an interchain disulfide bond)
• Nível de qualidade: 100
• solubilidade: H₂O: soluble
• temperatura de armazenamento: 2-8°C
• Informações sobre genes: Clostridium tetani E88 ... tetX(1061100)

Descrição

Descrição geral

Tetanus toxin is also known as tetanospasmin. It is a 150-kDa protein,[1] produced by Clostridium tetani.[2] This protein consists of a light and a heavy chain.[3]

Aplicação

Tetanus toxin from Clostridium tetani has been used as the exocytosis inhibitor/Golgi-dependent secretion to examine whether secretory carrier membrane protein (SCAMP5) stimulated α-synuclein secretion via the conventional Golgi-dependent pathway.[4] It has also been used in peripheral blood mononuclear cells (PBMCs) culture for T cell proliferation screening.[5]

Tetanus toxin from Clostridium tetani has been used to inhibit vesicular release in Pannexin 1-transfected Neuro2A cells¹.

Ações bioquímicas/fisiológicas

Binds to polysialogangliosides acceptors on surface of all peripheral presynaptic nerve terminals. Internalized by receptor-mediated endocytosis and carried to spinal cord and brain by transsynaptic retrograde transport. Blocks the release of glycine from inhibitory interneurons of the spinal cord. Cleaves synaptobrevin (VAMP-2), and blocks synaptic vesicle exocytosis in vivo.

Binds to polysialogangliosides acceptors on surface of all peripheral presynaptic nerve terminals; blocks the release of glycine from inhibitory interneurons of the spinal cord; cleaves synaptobrevin (VAMP-2); blocks synaptic vesicle exocytosis in vivo.

Tetanus toxin can cause spastic paralysis.[3]

Nota de preparo

Purified from cultures of Clostridium tetani by a modification of the method of Robinson, et al.

Reconstituição

Each vial, when reconstituted with 100 μL of sterile purified water, contains 25 μg of tetanus toxin in 20 mM HEPES, pH 7.4 with 1.25% lactose.

Nota de análise

Two bands by SDS-PAGE under reducing conditions (100 kDa heavy chain and 50 kDa light chain).

Informações de segurança

• Pictogramas: GHS06,GHS08
• Palavra indicadora: Danger
• Frases de perigo: H300 + H330,H370
• Declarações de precaução: P260 - P264 - P270 - P271 - P284 - P304 + P340 + P310
• Classificações de perigo: Acute Tox. 1 Oral - Acute Tox. 2 Inhalation - STOT SE 1
• Órgãos-alvo: Central nervous system
• Código de classe de armazenamento: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges