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T2507

Sigma-Aldrich

Triosephosphate Isomerase from baker′s yeast (S. cerevisiae)

Type I, ammonium sulfate suspension, ~10,000 units/mg protein

Sinônimo(s):

D-Glyceraldehyde-3-phosphate ketol-isomerase, TPI

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5 MG
R$ 2.131,00
10 MG
R$ 3.946,00
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R$ 8.516,00

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5 MG
R$ 2.131,00
10 MG
R$ 3.946,00
25 MG
R$ 8.516,00

About This Item

Número CAS:
9023-78-3
Número da licença da enzima:
5.3.1.1 (BRENDA, IUBMB)
Número CE:
232-774-2
Número MDL:
MFCD00082080
Código UNSPSC:
12352204
NACRES:
NA.54

R$ 2.131,00

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tipo

Type I

Nível de qualidade

200

Formulário

ammonium sulfate suspension

atividade específica

~10,000 units/mg protein

peso molecular

53 kDa by equilibrium centrifugation

atividade externa

pyruvate kinase, lactic dehydrogenase, 3-phosphoglyceric phosphokinase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase <0.01%

temperatura de armazenamento

2-8°C

Categorias relacionadas

Aplicação

Triosephosphate isomerase has been used in a study to assess differential expression of fourteen proteins of uveal melanoma. [1] Triosephosphate isomerase has also been used in a study to investigate the use of sigmoid pH gradients in free-flow isoelectric focusing of human endothelial cell proteins. [2]

Definição da unidade

One unit will convert 1.0 μmole D-glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 at 25 °C.

forma física

Crystalline suspension in 2.7 M (NH4)2SO4, 0.5 mM EDTA, pH 6.5

Nota de análise

Protein determined by biuret.

Outras notas

Since the assay of this enzyme seems to be sensitive to minor variables, packages are dispensed per mg protein rather than in units.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

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Certificados de análise (COA)

Lot/Batch Number
036H8025
060H8110

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Xiao Ma et al.
Electrophoresis, 33(9-10), 1349-1355 (2012-06-01)
Prefractionation of proteins enhances the resolution of proteome analysis of whole cells. Free-flow electrophoresis (FFE) provides a useful step in various prefractionation protocols, since matrix-free isoelectric focusing (FF-IEF) performed in this machine enables the enrichment of large, easily absorbable, sensitive
Annett Linge et al.
Investigative ophthalmology & visual science, 53(8), 4634-4643 (2012-05-10)
To compare the proteomic profiles of two categories of primary uveal melanoma tissue samples; those from patients who have subsequently developed metastatic disease and those who have not. Two-dimensional difference gel electrophoresis (2D DIGE) was performed on 25 uveal melanoma
Xiaochen He et al.
Journal of cellular physiology, 237(8), 3317-3327 (2022-05-28)
Hypertension is an important risk factor in the pathogenesis of diastolic dysfunction. Growing evidence indicates that glucose metabolism plays an essential role in diastolic dysfunction. TP53-induced glycolysis and apoptosis regulator (TIGAR) has been shown to regulate glucose metabolism and heart
Rebecca Blomberg et al.
Nature, 503(7476), 418-421 (2013-10-18)
Linus Pauling established the conceptual framework for understanding and mimicking enzymes more than six decades ago. The notion that enzymes selectively stabilize the rate-limiting transition state of the catalysed reaction relative to the bound ground state reduces the problem of
Rongchang Yang et al.
Experimental parasitology, 137, 46-52 (2013-12-21)
A novel quantitative PCR (qPCR) for Giardia at the glutamate dehydrogenase (gdh) locus was developed and validated. The qPCR was used to screen a total of 3412 lamb faecal samples collected from approximately 1189 lambs at three sampling periods (weaning
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How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

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