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SRP6410

Sigma-Aldrich

ApoA-1 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

Sinônimo(s):

Apolipoprotein A-I, MGC117399

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About This Item

Código UNSPSC:
12352202
NACRES:
NA.32

fonte biológica

human

recombinante

expressed in HEK 293 cells

etiqueta

6-His tagged (C-terminus)

Ensaio

≥95% (SDS-PAGE)

forma

lyophilized

peso molecular

calculated mol wt 29 kDa
observed mol wt 26-30 kDa (DTT-reduced. Asp 25 is the predicted N-terminal.)

embalagem

pkg of 10 μg

Impurezas

<1 EU/μg endotoxin (LAL test)

nº de adesão UniProt

P02647

Condições de expedição

wet ice

temperatura de armazenamento

−20°C

Informações sobre genes

human ... ApoA1(335)

Descrição geral

ApoA1 is also known as apolipoprotein A-I, ApoA-I , and is the major protein component of high density lipoprotein (HDL) in plasma. It has a specific role in lipid metabolism. Chylomicrons secreted from the intestinal enterocyte also contain ApoA1 but it is quickly transferred to HDL in the bloodstream. The protein promotes cholesterol efflux from tissues to the liver for excretion. It is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. ApoA-I was also isolated as a prostacyclin (PGI2) stabilizing factor, and thus may have an anticlotting effect. Defects in the gene encoding it are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. In addition, it has been shown that ApoA1 is implicated in the anti-endotoxin function of HDL via interaction with lipopolysaccharide or endotoxin.

forma física

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstituição

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

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Anastasia M Ravodina et al.
ACS omega, 5(36), 23289-23298 (2020-09-22)
Here, we report a nanoparticle-based probe that affords facile cell labeling with cholesterol in cholesterol efflux (CE) assays. This probe, called ezFlux, was optimized through a screening of multiple nanoformulations engineered with a Förster resonance energy transfer (FRET) reporter. The

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