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SRP3134

Sigma-Aldrich

p16-INK-4a

recombinant, expressed in E. coli, lyophilized, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for mammalian cell culture

Sinônimo(s):

CDK4I, Cyclin-dependent kinase 4 inhibitor A, Cyclin-dependent kinase inhibitor 2A, Multiple tumor suppressor 1, p16-INK4, p16INK4A

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About This Item

Código UNSPSC:
12352202
NACRES:
NA.32

product name

p16-INK-4a human, recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

fonte biológica

human

recombinante

expressed in E. coli

Ensaio

≥95% (HPLC)
≥95% (SDS-PAGE)

forma

lyophilized

peso molecular

16.5 kDa

embalagem

pkg of 20 μg

técnica(s)

cell culture | mammalian: suitable

Impurezas

<0.1 EU/μg endotoxin, tested

cor

off-white to yellow

nº de adesão UniProt

P42771

Condições de expedição

wet ice

temperatura de armazenamento

−20°C

Informações sobre genes

human ... CDKN2A(1029)

Descrição geral

p16-INK4a, which is also known as cyclin dependent kinase inhibitor 2A (CDKN2A), is a nuclear protein. The gene encoding this protein is localized on human chromosome 9p21, with three exons. Recombinant p16-INK4a is a 16.5kDa protein containing 156 amino acid residues.

Ações bioquímicas/fisiológicas

Cyclin dependent kinase inhibitor 2A (CDKN2A) or p16-INK4a regulates the cell cycle by inhibiting cyclin dependent kinase-4 (CDK4) and CDK6. It inhibits CDK activity by binding to the CDK molecules in a manner that interferes with their ability to interact with cyclin D. This activity has the effect of suppressing tumor formation and growth, and of inducing replicative senescence in various normal cells, including stem cells. The expression of p16-INK4a steadily increases with age and tends to accumulate in stem cell compartments. The deletion, rearrangement, or mutation of the p16-INK4a gene is frequently found in melanomas as well as in certain other types of cancer. The protein is downregulated in bladder cancer.

Sequência

MEPAAGSSME PSADWLATAA ARGRVEEVRA LLEAGALPNA PNSYGRRPIQ VMMMGSARVA ELLLLHGAEP NCADPATLTR PVHDAAREGF LDTLVVLHRA GARLDVRDAW GRLPVDLAEE LGHRDVARYL RAAAGGTRGS NHARIDAAEG PSDIPD

forma física

Lyophilized from 1 x PBS + 1.0 mM DTT, pH 7.2.

Reconstituição

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Pictogramas

Exclamation mark
GHS07

Palavra indicadora

Warning

Frases de perigo

H319

Classificações de perigo

Eye Irrit. 2

Código de classe de armazenamento

13 - Non Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Visite a Biblioteca de Documentos

Prognostic and Clinicopathological Significance of Downregulated p16 Expression in Patients with Bladder Cancer: A Systematic Review and Meta-Analysis.
Gan X
Disease Markers, 5259602-5259602 (2016)
Prevalence of p16 and CDK4 germline mutations in 48 melanoma-prone families in France. The French Familial Melanoma Study Group.
Soufir N
Human Molecular Genetics, 7(2), 209-216 (1998)
Immunohistological Expression of p16INK4a is Commonly Present Both in Benign and Malignant Sweat Gland Neoplasias.
Tsujita J
Fukuoka Igaku Zasshi = Hukuoka Acta Medica, 106(12), 323-329 (2015)
Jinsuke Nishino et al.
Cell, 135(2), 227-239 (2008-10-30)
Stem cells persist throughout life in diverse tissues by undergoing self-renewing divisions. Self-renewal capacity declines with age, partly because of increasing expression of the tumor suppressor p16(Ink4a). We discovered that the Hmga2 transcriptional regulator is highly expressed in fetal neural
A Okamoto et al.
Proceedings of the National Academy of Sciences of the United States of America, 91(23), 11045-11049 (1994-11-08)
Cell cycle arrest at the G1 checkpoint allows completion of critical macromolecular events prior to S phase. Regulators of the G1 checkpoint include an inhibitor of cyclin-dependent kinase, p16INK4; two tumor-suppressor proteins, p53 and RB (the product of the retinoblastoma-susceptibility

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