SRP0213

PTP1B (1-321)/PTPN1 Active from mouse

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• Sinônimo(s): PTPN1, Protein tyrosine phosphatase, non-receptor type 1
• Código UNSPSC: 12352200
• NACRES: NA.32

Propriedades

• fonte biológica: mouse
• recombinante: expressed in E. coli
• Ensaio: ≥90% (SDS-PAGE)
• forma: aqueous solution
• peso molecular: 63.3 kDa
• embalagem: pkg of 20 μg
• condição de armazenamento: avoid repeated freeze/thaw cycles
• concentração: >0.02 mg/mL
• nº de adesão NCBI: NM_011201.3
• nº de adesão UniProt: P35821
• Condições de expedição: dry ice
• temperatura de armazenamento: −70°C
• Informações sobre genes: human ... PTPN1(5770)

Descrição

Descrição geral

Protein tyrosine phosphatase, Catalytic domain, (amino acids 1-321), with N-terminal GST tag, MW = 63.3 kDa, expressed in an E. coli expression system.

Aplicação

Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.

Definição da unidade

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

forma física

Formulation: recombinant proteins were eluted in 25 mM Tris-HCL, 75 mM NaCl, pH 8.0, 0.05% Tween, 10 mM glutathione, 1 mM DTT and 50% glycerol.

Nota de preparo

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.