SAB4200075

Anti-VAC14 (C-terminal) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• Sinônimo(s): Anti-ArPIKfyve, Anti-TAX1-binding protein 2, Anti-TAX1BP2, Anti-TAX1BP2 TAX-reactive protein x, Anti-TRX
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• conjugado: unconjugated
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• Formulário: buffered aqueous solution
• peso molecular: antigen ~82 kDa
• reatividade de espécies: rat, mouse, human
• embalagem: antibody small pack of 25 μL
• concentração: ~1.5 mg/mL
• técnica(s): immunoprecipitation (IP): 10-15 μg using A431 cell lysates; indirect immunofluorescence: 8-16 μg/mL using HeLa cells; western blot: 1-2 μg/mL using extracts of mouse brain (S1 fraction) or rat brain (S1 fraction)
• nº de adesão UniProt: Q08AM6
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... VAC14(55697) ; rat ... Vac14(307842)

Descrição

Descrição geral

VAC14 gene is mapped to human chromosome 16q22.1-q22.2. VAC14 is a scaffold protein and is a component of the PIKfyve protein kinase complex.

Especificidade

Anti-VAC14 (C-terminal) specifically recognizes human, mouse, and rat VAC14.

Aplicação

Anti-VAC14 (C-terminal) antibody produced in rabbit has been used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

Ações bioquímicas/fisiológicas

Mutation in VAC14 gene leads to the development of retinitis pigmentosa (RP).

VAC14 is a regulator of PIP5K3/PIKfyve, a dual specificity kinase.

VAC14 phosphorylates PtdIns(3)P to generate the housekeeping phospholipid PtdIns(3,5)P₂. It associates with and upregulate PIKfyve phosphoinositide-5-kinase activity. VAC14-PIP5K3- PtdIns(3,5)P₂ pathway is thought to be physiologically linked to insulin activation of glucose transport into the cell. Knockout of the VAC14 gene in mice results in massive neurodegeneration and particularly affected are neurons in the midbrain and of the peripheral sensory system.

forma física

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Armazenamento e estabilidade

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable