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S5171

Sigma-Aldrich

Human Fibronectin

formulated with recombinant human fibronectin fragment III-C expressed in E. coli and fibronectin from human plasma, liquid, 2 mg/mL, suitable for cell culture

Sinônimo(s):

sFN

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0.5 MG
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About This Item

Número MDL:
MFCD00284706
Código UNSPSC:
12352202
NACRES:
NA.75

R$ 2.655,00

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Nome do produto

Superfibronectin from human plasma, solution, BioReagent, suitable for cell culture

fonte biológica

human

Nível de qualidade

200

recombinante

expressed in E. coli

linha de produto

BioReagent

Formulário

solution

peso molecular

~235-270 kDa

embalagem

pkg of 5 mg

técnica(s)

cell culture | mammalian: suitable

cobertura de superfície

1 μg/cm2

nº de adesão UniProt

P02751

Condições de expedição

ambient

temperatura de armazenamento

2-8°C

Informações sobre genes

human ... FN1(2335)

Categorias relacionadas

Aplicação

Superfibronectin from human plasma dramatically enhances the adhesive properties of fibronectin and suppresses cell migration.[1][2] Improved results have been shown CHO cells, IMR-90 fibroblasts, T47D breast carcinoma cells, and UCLA-P lung carcinoma cells. Additional applications include epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, and endothelial cells. It is recommended for use as a cell culture substratum at 1 μg/cm2.

Componentes

Superfibronectin is formulated with recombinant, human fibronectin fragment III-C expressed in E. coli and fibronectin from human plasma. The recombinant fibronectin includes a new region, type III, cloned from human placenta cDNA, that is newly reported to participate in matrix formation by inducing spontaneous disulfide crosslinking of fibronectin molecules into multimers. The product resembles the in vivo matrix form of fibronectin.

Atenção

Superfibronectin should be stored at 2-8°C and diluted immediately prior to use. Do not freeze this product.

Nota de preparo

As supplied, this product is a 2 mg protein/mL solution in 0.05 M Tris buffered saline. Before coating a 96 well plate, it is recommended to dilute t he product with sterile PBS to a concentration of 5 μg/mL and add 100 μl/well. These plates should then be incubated for 2 hours at 37°C and washed twice with 100 μl of PBS. The source material has been tested for HBsAg and HIV antibodies.

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

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Certificados de análise (COA)

Lot/Batch Number
0000388233
0000388233
0000198891
11180741
0000117157

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Gentle cell handling with an ultra-slow instrument: creep-manipulation of cells
Thielecke H, et al.
Microscopica Acta, 11(11), 1230-1241 (2005)
Regulated GPCR trafficking to the plasma membrane: general issues and the CCR5 chemokine receptor example
Giornale di Psichiatria e di Neuropatologia (2012)
N Okada et al.
The Journal of biological chemistry, 272(43), 26978-26984 (1997-10-27)
The pathogenic Gram-positive bacterium Streptococcus pyogenes (group A streptococcus) binds to fibronectin via protein F. In this study, we have investigated the binding properties of protein F to various multimeric tissue forms of fibronectin that appear on cell surfaces and
Florian Weinberg et al.
International journal of molecular sciences, 21(23) (2020-12-03)
The epidermal growth factor receptor HER2 is overexpressed in 20% of breast cancer cases. HER2 is an orphan receptor that is activated ligand-independently by homodimerization. In addition, HER2 is able to heterodimerize with EGFR, HER3, and HER4. Heterodimerization has been
A Morla et al.
Nature, 367(6459), 193-196 (1994-01-13)
Fibronectin is an extracellular matrix protein that is important in development, wound healing and tumorigenesis. In the blood it is dimeric, but in tissues forms disulphide crosslinked fibrils. Here we show that a fragment from the first type-III repeat of
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Protocolos

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