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Sigma-Aldrich

Thrombin CleanCleave Kit

Sinônimo(s):

Thrombin Cleavage Kit

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1 KIT
R$ 1.590,00

R$ 1.590,00


Previsão de entrega em31 de março de 2025


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1 KIT
R$ 1.590,00

About This Item

Código UNSPSC:
12352200
NACRES:
NA.56

R$ 1.590,00


Previsão de entrega em31 de março de 2025


Solicite uma grande encomenda

Formulário

suspension

Nível de qualidade

peso molecular

37 kDa

técnica(s)

protein purification: suitable

Condições de expedição

wet ice

temperatura de armazenamento

2-8°C

Descrição geral

The Thrombin CleanCleave Kit contains a 50% suspension fo thrombin-agarose produced by immobilizing bovine thrombin and is designed for cleavage of recombinant fusion proteins

Aplicação

For cleaving a tag from a recombinant fusion protein which contains the thrombin recognition sequence. Thrombin-agarose is compatible with recombinant proteins expressed in various types of expression systems.

Características e benefícios

  • Fast, efficient cleavage in as little as 2 hours
  • Thrombin is covalently bound to agarose for easy removal.
  • The robust cleavage reaction is effective at temperatures from 4 °C to 37 °C and over a wide range of pH and ionic strengths.
  • Cleave tags even in the presence of 0.1% Triton, 1 M urea or 5 mM EDTA
  • Thrombin-agarose is reusable.

Quantidade

200 μl of a 50% slurry of thrombin-agarose cleaves >85% of 1 mg of fusion protein.

Informações legais

CleanCleave is a trademark of Sigma-Aldrich Co. LLC
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Somente componentes do kit

Nº do produto
Descrição

  • Thrombin Cleavage Buffer, 10× 10 mL

produto relacionado

Nº do produto
Descrição
Preços

Código de classe de armazenamento

10 - Combustible liquids

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Artigos

This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.

Protocolos

This page how to remove GST tags by enzymatic cleavage using Cytiva products.

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