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Sigma-Aldrich

TruPAGE Precast Gels

4-20%, 10 x 8cm, 17-well

Sinônimo(s):

Precast Gels for SDS-PAGE, Precast Polyacrylamide Gels

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About This Item

Código UNSPSC:
41105300
NACRES:
NB.22

descrição

for use with Bio-Rad Mini-PROTEAN™ Tetra Cell, Mini-PROTEAN Dodeca™, Mini-PROTEAN II and 3 cells reverse device gasket to flat side

prazo de validade

24 mo. at 2‑8 °C (from the date of manufacture)

embalagem

box of 10 ea

concentração

4-20% polyacrylamide

cassete L × C

10 cm × 8 cm

espessura do gel

1 mm

tamanho do poço × volume

17 wells × 17 μL

compatibilidade

for use with Hoefer Mighty Small II and Tall Mighty Small (SE 260 and SE280)

temperatura de armazenamento

2-8°C

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Aplicação

TruPAGE Precast Gels 4-20% have been used to separate proteins through sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by Western blotting.

Características e benefícios

TruPAGE SDS-PAGE Precast Gels provide improved protein resolution, tear resistance, and an extended shelf-life. TruPAGE gels are formulated with Triethanolamine (TEA)-Tricine, featuring a neutral pH running environment that helps prevents protein modification during the course of the experiment.
The precast polyacrylamide gels come in two gel cassette sizes (10 x 10 cm and 10 x 8 cm) for increased equipment compatibility. TruPAGE gels have extra tall wells to prevent lane-to-lane overflow and come in two well formats (12-well and 17-well). TruPAGE gels are available in several different gel concentrations (10%, 12%, 4-8%, 4-12% and 4-20%) to provide desired resolution of proteins of any size. Please refer to the migration chart to select the appropriate precast gel and running buffer combination for your experimental needs. Only use TruPAGE formulated buffers when running TruPAGE gels to ensure optimal results and reproducibility. Please review the TruPAGE Technical Bulletin for buffer formulation recipes and general running guidelines.

  • Long Shelf Life - 2 years from the date of manufacture when stored properly.
  • High Performance - Extended full length resoling gels provides improved resolution.
  • Durable - Stronger than standard polyacrylamide gels, preventing tearing during post-electrophoresis processing.
  • Extra Tall Wells - Eliminate sample spill over and accommodate larger sample loading volumes with well teeth that protrude above the back plate of the gel cassette.
  • Excellent Compatibility - TruPAGE gels are compatible with the popular gel running equipment, including Sigma-Aldrich′s Dual Run Tank (Z741768) and other manufacturers like Life Technologies, Bio-Rad®, Lonza®, Hoefer®, CBS Scientific as well as others, allowing you to continue using the equipment you already own.
  • Fast - Run times as low as 30 minutes.
  • Great Value - Gels and buffers offer a cost-saving alternative without sacrificing performance and experimental integrity.
TruPAGE Precast Gels Migration Chart
There is no comb to remove, which prevents torn or bent wells. Instead, the cassettes lock well fingers in place. There is also no tape to remove.

Informações legais

Bio-Rad is a registered trademark of Bio-Rad Laboratories, Inc.
Hoefer is a registered trademark of Hoefer, Inc.
Lonza is a registered trademark of Lonza Group Ltd.
TruPAGE is a trademark of Sigma-Aldrich Co. LLC

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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Visite a Biblioteca de Documentos

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Wang Z, et al.
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Comparison of the effects of metformin on MDA-MB-231 breast cancer cells in a monolayer culture and in tumour spheroids as a function of nutrient concentrations.
Bizjak M, et al.
bioRxiv, 351742-351742 (2018)

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