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P3803

Sigma-Aldrich

Phenol:Chloroform:Isoamyl Alcohol 25:24:1, Saturated with 10mM Tris, pH 8.0, 1mM EDTA

for molecular biology

Sinônimo(s):

DNA extraction

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About This Item

Código UNSPSC:
12190000
NACRES:
NA.52

grau

for molecular biology

Nível de qualidade

200

Ensaio

≥99.0%

forma

liquid

pH

6.5-6.9(pH of phenolic phase)

aplicação(ões)

agriculture

temperatura de armazenamento

2-8°C

Aplicação

Removal of proteins from nucleic acids can be achieved by extraction with phenol:chloroform: isoamyl alcohol solutions. For crude mixtures of nucleic acids, digestion with a broad range proteolytic enzyme prior to the phenol:chloroform extraction may be beneficial. Although proteins are efficiently denatured by phenol, RNase activity is not completely inhibited. Therefore, a small amount of isoamyl alcohol is added to further ensure the deactivation of RNase activity. Please see Product Information Sheet for the protocol for removal of proteins from nucleic acids.

Advertência

If phenol solutions come in contact with skin, burns will occur quickly. It is, therefore, required that the user wear appropriate laboratory clothing including gloves and protective eyewear. In case of direct skin contact, rinse extensively with water and seek medical attention if necessary.

Outras notas

This product has a shelf life of two years.

Produtos recomendados

For DNA extraction, the pH of the phenol phase can be adjusted to 8.0 by equilibration with tris buffer. Sigma offers product P2069 specifically for DNA extractions. This product includes equilibration buffer for the convenience of adjusting the pH.

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Palavra indicadora

Danger

Classificações de perigo

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2 - STOT SE 3

Órgãos-alvo

Central nervous system, Liver,Kidney, Nervous system,Kidney,Liver,Skin

Código de classe de armazenamento

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Bizunesh Abere et al.
mBio, 11(6) (2020-12-17)
Viral noncoding RNAs have acquired increasing prominence as important regulators of infection and mediators of pathogenesis. Circular RNAs (circRNAs) generated by backsplicing events have been identified in several oncogenic human DNA viruses. Here, we show that Merkel cell polyomavirus (MCV)
Section 1 Isolation of nucleic acids
Isabel Ferrera
Biofouling, 88-88 (2014)
Tzemach Aouizerat et al.
Bio-protocol, 10(1), e3473-e3473 (2020-01-05)
Ancient fermented food has been studied mainly based on residue analysis and recipes and reconstruction attempts were performed using modern domesticated yeast. Furthermore, microorganisms which participated in fermentation were studied using ancient-DNA techniques. In a recent paper, we presented a
Jeong-Kui Ku et al.
Annals of translational medicine, 8(6), 314-314 (2020-05-02)
The manufacturing of the demineralized dentin matrix (DDM) has been proven to extensively reduce the presence of human hepatitis B viral DNA (HBV DNA). This study measured and compared HBV DNA in fresh dentin to that in gamma radiation (GR)-sterilized
Exogenous controls increase negative call veracity in multiplexed, quantitative PCR assays for Phakopsora pachyrhizi
James Haudenshield
Plant Disease, 95.3, 343-352 (2011)
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