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Documentos Principais

N3014

Sigma-Aldrich

β-Dinucleotídeo de nicotinamida adenina

suitable for cell culture, ≥96.5% (HPLC), ≥96.5% (spectrophotometric assay), from yeast

Sinônimo(s):

β-DPN, β-NAD, Coenzima 1, Cozimase, DPN, NAD, Nadide, Nucleotídeo de difosfopiridina

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About This Item

Fórmula empírica (Notação de Hill):
C21H27N7O14P2 · xH2O
Número CAS:
Peso molecular:
663.43 (anhydrous basis)
Número MDL:
Código UNSPSC:
41106305
eCl@ss:
39200202
ID de substância PubChem:
NACRES:
NA.55
Preço e disponibilidade não estão disponíveis no momento.

fonte biológica

yeast

Nível de qualidade

Ensaio

≥96.5% (HPLC)
≥96.5% (spectrophotometric assay)

Formulário

powder

peso molecular

Mw 663.43 g/mol

técnica(s)

cell culture | mammalian: suitable

Impurezas

≤3% Acetone

cor

white to off-white

solubilidade

H2O: 50 mg/mL

temperatura de armazenamento

−20°C

cadeia de caracteres SMILES

NC1=NC=NC2=C1N=CN2.O[C@@H]3[C@@H](COP(O)(OP(OC[C@@H](O4)[C@@H](O)[C@@H](O)[C@H]4[N+]5=CC=CC(C(N)=O)=C5)([O-])=O)=O)OC[C@@H]3O

InChI

1S/C21H27N7O14P2/c22-17-12-19(25-7-24-17)28(8-26-12)21-16(32)14(30)11(41-21)6-39-44(36,37)42-43(34,35)38-5-10-13(29)15(31)20(40-10)27-3-1-2-9(4-27)18(23)33/h1-4,7-8,10-11,13-16,20-21,29-32H,5-6H2,(H5-,22,23,24,25,33,34,35,36,37)/t10-,11-,13-,14-,15-,16-,20-,21-/m1/s1

chave InChI

BAWFJGJZGIEFAR-NNYOXOHSSA-N

Informações sobre genes

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Aplicação


  • Spectroelectrochemical Enzyme Sensor System for Acetaldehyde Detection in Wine.: This study explores the development of a novel sensor system using β-Nicotinamide adenine dinucleotide hydrate to detect acetaldehyde in wine, enhancing the accuracy of wine quality control (Ibáñez et al., 2022).

  • Screening of Lactate Dehydrogenase Inhibitor from Bioactive Compounds in Natural Products by Electrophoretically Mediated Microanalysis.: The research utilizes β-Nicotinamide adenine dinucleotide hydrate to screen potential lactate dehydrogenase inhibitors, which could lead to new therapeutic agents for diseases such as cancer (Li et al., 2021).

  • Combination of NAD(+) and NADPH Offers Greater Neuroprotection in Ischemic Stroke Models by Relieving Metabolic Stress.: This research highlights the neuroprotective effects of NAD+ and NADPH in stroke models, emphasizing the potential therapeutic applications of β-Nicotinamide adenine dinucleotide hydrate in neuroprotection (Huang et al., 2018).

Ações bioquímicas/fisiológicas

Aceptor de elétrons

Embalagem

Embalado com base no peso sólido.

Outras notas

Meets or exceeds NRC specifications.
Esta é a forma comum da NAD.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Ana P Gomes et al.
Cell, 155(7), 1624-1638 (2013-12-24)
Ever since eukaryotes subsumed the bacterial ancestor of mitochondria, the nuclear and mitochondrial genomes have had to closely coordinate their activities, as each encode different subunits of the oxidative phosphorylation (OXPHOS) system. Mitochondrial dysfunction is a hallmark of aging, but
Jeerus Sucharitakul et al.
The Journal of biological chemistry, 288(49), 35210-35221 (2013-10-17)
3-Hydroxybenzoate 6-hydroxylase (3HB6H) from Rhodococcus jostii RHA1 is an NADH-specific flavoprotein monooxygenase that catalyzes the para-hydroxylation of 3-hydroxybenzoate (3HB) to form 2,5-dihydroxybenzoate (2,5-DHB). Based on results from stopped-flow spectrophotometry, the reduced enzyme-3HB complex reacts with oxygen to form a C4a-peroxy
Christian Dölle et al.
The FEBS journal, 280(15), 3530-3541 (2013-04-27)
Mitochondrial metabolism is intimately connected to the universal coenzyme NAD. In addition to its role in redox reactions of energy transduction, NAD serves as substrate in regulatory reactions that lead to its degradation. Importantly, all types of the known NAD-consuming
Brian J North et al.
The EMBO journal, 33(13), 1438-1453 (2014-05-16)
Mice overexpressing the mitotic checkpoint kinase gene BubR1 live longer, whereas mice hypomorphic for BubR1 (BubR1(H/H)) live shorter and show signs of accelerated aging. As wild-type mice age, BubR1 levels decline in many tissues, a process that is proposed to
Anna Ferretta et al.
Biochimica et biophysica acta, 1842(7), 902-915 (2014-03-04)
Mitochondrial dysfunction and oxidative stress occur in Parkinson's disease (PD), but the molecular mechanisms controlling these events are not completely understood. Peroxisome proliferator-activated receptor-gamma coactivator-1α (PGC-1α) is a transcriptional coactivator known as master regulator of mitochondrial functions and oxidative metabolism.

Questions

  1. I would like to know, how much stable is this drug once I dilute the drug ( Beta nicotinamide adenine dinucleotide hydrate, N3014-5g) in PBS or H2O. Can I store this drug at -20 degrees once I make the solution? and for how long? can i freeze thaw it?

    1 answer
    1. Aqueous solutions between pH 2 - 6, stored as single-use aliquots at -70 °C, are stable for at least 6 months. Please see the link below to review the product data sheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/345/899/n3014pis.pdf

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