MTOX1092P24
MRP1 Knockout Caco-2 Cells
human cervix, Epithelial
Sinônimo(s):
MRP1 Knockout Caco-2 Cells
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About This Item
Código UNSPSC:
12352200
NACRES:
NA.81
Produtos recomendados
product name
MRP1 Knockout Caco-2 Cells, one assay ready, 24 well plate
fonte biológica
human colon
descrição
one assay ready, 24 well plate
forma
liquid
morfologia
Epthelial
técnica(s)
drug transporter assay: suitable
permeability assay: suitable
aplicação(ões)
ADME/TOX
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Descrição geral
The C2BBe1 cells, a subclone of Caco-2 cells, correspond to ATCC Cat. No. CRL-2102. The MRP1 knockout C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years) with functional knockout of the MRP1 efflux transporter.
The three week production lead time begins on the Monday following a purchase, in the third week the cells are shipped overnight for receipt on Tuesday or Wednesday. As a biologic product that′s shipped at room temperature the cells must be processed immediately upon receipt.
The three week production lead time begins on the Monday following a purchase, in the third week the cells are shipped overnight for receipt on Tuesday or Wednesday. As a biologic product that′s shipped at room temperature the cells must be processed immediately upon receipt.
Aplicação
The following posters and articles demonstrate how Caco-2 cells can be used for cell based assays:
Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases
Comparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 Genes
Caco-2 Transporter Knockout Cell Based Assays
Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases
Comparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 Genes
Caco-2 Transporter Knockout Cell Based Assays
Características e benefícios
The Caco-2 subclone, C2BBe1 cells, are ideal for transporter analysis as they express multiple transporters, are human derived and grow in a homogenous monolayer that forms tight juntions which is necessary for efflux ratio analysis. Other benefits include:
- A functional knockout of the MRP1 gene eliminates the reliance on chemical inhibitors to determine if a compound is an MRP1 substrate
- The 24 well Transwell format enables the MRP1 knockout cells to be included in standard drug transporter protocols
- Human assay with no interference from animal inhibitors
- Overcome the limitations of RNAi and knockdown cell lines that arise from remaining transporter functionality
- A functional knockout of the MRP1 gene eliminates the reliance on chemical inhibitors to determine if a compound is an MRP1 substrate
- The 24 well Transwell format enables the MRP1 knockout cells to be included in standard drug transporter protocols
- Human assay with no interference from animal inhibitors
- Overcome the limitations of RNAi and knockdown cell lines that arise from remaining transporter functionality
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Descrição
Preços
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
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P Artursson
Journal of pharmaceutical sciences, 79(6), 476-482 (1990-06-01)
A human intestinal cell line, Caco-2, was used as a model to study the passive diffusion of drugs across intestinal epithelium. The cells formed continuous monolayers when grown on permeable filters of polycarbonate. After 10 days in culture, the monolayers
M J Briske-Anderson et al.
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.), 214(3), 248-257 (1997-03-01)
The Caco-2 cell line is used by many investigators as a model of the intestinal epithelium to study nutrient uptake and transport. Our goal was to create an awareness of inherent variabilities in the Caco-2 cell line which may influence
Mark I Kaldas et al.
The Journal of pharmacy and pharmacology, 55(3), 307-312 (2003-05-02)
Resveratrol is a dietary constituent suggested to have protective effects against cancer as well as cardiovascular disease. The purpose of the study was to learn whether this agent could be absorbed in man and enter the systemic circulation. This was
S Yee
Pharmaceutical research, 14(6), 763-766 (1997-06-01)
To evaluate and optimize the use of Caco-2 cell monolayers to predict the in vivo absorption of a broad range of compounds in man. Caco-2 cells are derived from human adenocarcinoma colon cells and spontaneously differentiate when grown on porous
V Pade et al.
Journal of pharmaceutical sciences, 87(12), 1604-1607 (1999-04-03)
The objective of this investigation was to establish a relationship between drug permeability and solubility in vitro and the extent of drug absorption in humans. We selected drugs with varying permeabilities and solubilities with the aim of establishing a relationship
Artigos
Application note on Drug transport assays in a 96-well system using Millicell-96 System from Millipore.
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